Organization of oligodendroglial membrane sheets. I: Association of myelin basic protein and 2',3'-cyclic nucleotide 3'-phosphohydrolase with cytoskeleton.

Journal of Neuroscience Research
C A Dyer, J A Benjamins

Abstract

Membrane sheets elaborated by cultured murine oligodendroglia provide a unique system for examining associations between myelin proteins and cytoskeletal elements. Interactions can be observed and manipulated more readily than in the multilamellar myelin membrane in vivo. Immunocytochemical staining of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) shows that it is distributed diffusely in some regions of membrane sheets, but colocalized with tubulin in lacy networks and major veins in other regions. Staining with phalloidin also reveals two distributions of F-actin: 1) small aggregates within the diffuse CNPase regions and 2) filaments colocalized with tubulin and CNPase in the lacy networks and veins. Application of colchicine at 10 micrograms/ml for 4 hr disrupts microtubular structures in the lacy network, while those in major veins remain intact. This suggests that microtubules in the lacy network are treadmilling more rapidly than those in the major veins. The distribution of CNPase and F-actin is not altered under these conditions. In contrast, cytochalasin B disrupts F-actin, microtubules, and CNPase in the lacy networks, indicating that cross-linking between these three proteins is disrupted. Both colchicine and ...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D BrayG Shaw
Sep 1, 1978·The Journal of Cell Biology·L M Griffith, T D Pollard
Oct 1, 1988·Journal of Neuroscience Research·A N Verity, A T Campagnoni
Apr 1, 1989·Journal of Neuroscience Research·R Wilson, P J Brophy
Jun 19, 1986·Nature·J R BamburgK Chapman
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B D TrappJ Griffin
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G S BloomR B Vallee
Nov 1, 1982·The Journal of Cell Biology·D R ColmanD D Sabatini
Jun 1, 1980·Cell·S MacLean-Fletcher, T D Pollard

❮ Previous
Next ❯

Citations

Oct 26, 1999·Journal of Neuroscience Research·G L BoccaccioD R Colman
Oct 10, 2001·Journal of Neuroscience Research·P C BarradasM S De Freitas
Nov 1, 1994·Journal of Neuroscience Research·D A De Angelis, P E Braun
Dec 1, 1995·Journal of Neuroscience Research·C Richter-Landsberg, M Heinrich
Dec 1, 1992·Journal of Neurocytology·I D DuncanL Brignolo-Holmes
Dec 7, 2007·Journal of Molecular Neuroscience : MN·Christiane Richter-Landsberg
Jan 4, 2008·Journal of Molecular Neuroscience : MN·Olaf MaierWia Baron
Mar 3, 1994·Biochimica Et Biophysica Acta·J P VosB M Gadella
Dec 1, 1993·Trends in Neurosciences·P J BrophyD R Colman
Aug 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J Y LénaA Rabié
Feb 27, 2001·Brain Research. Molecular Brain Research·P KursulaA M Heape
Jan 13, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P C BarradasE G Moura
Jun 18, 2002·Journal of Neurochemistry·Christopher M TaylorSteven E Pfeiffer
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·P LoPrestiL I Binder
Jan 1, 1990·Annals of the New York Academy of Sciences·J A Benjamins, C A Dyer
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lillian S DeBruinGeorge Harauz
Jul 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bin ZhangFengyi Liang
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maurizio BifulcoJ Wolff
Jun 1, 1992·Progress in Neurobiology·Y Takahashi
Jun 29, 2004·Micron : the International Research and Review Journal for Microscopy·George HarauzChristophe Farès
Sep 22, 2015·Frontiers in Cellular Neuroscience·John-Paul Michalski, Rashmi Kothary
Jun 26, 2012·Biochimica Et Biophysica Acta·Daniel R KattnigDariush Hinderberger
Aug 1, 2011·Biomolecular Concepts·Allison Kraus, Marek Michalak
Jan 12, 2017·Cell Reports·Nicolas SnaideroMikael Simons
Sep 1, 1995·Medical Hypotheses·J KarthigasanD A Kirschner
Feb 5, 2000·Journal of Neuroscience Research·C Richter-Landsberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.