Organization of the mycobacterial cell wall: a nanoscale view.

Pflügers Archiv : European journal of physiology
David AlsteensYves F Dufrêne

Abstract

The biosynthesis of the Mycobacterium tuberculosis cell wall is targeted by some of the most powerful antituberculous drugs. To date, the molecular mechanisms by which these antibiotics affect the cell wall characteristics are not well understood. Here, we used atomic force microscopy - in three different modes - to probe the nanoscale surface properties of live mycobacteria and their modifications upon incubation with four antimycobacterial drugs: isoniazid, ethionamide, ethambutol, and streptomycine. Topographic imaging, combined with quantitative surface roughness analysis, demonstrated that all drugs induce a substantial increase of surface roughness to an extent that correlates with the localization of the target (i.e., synthesis of mycolic acids, arabinogalactans, or proteins). Chemical force microscopy with hydrophobic tips revealed that the structural alterations induced by isoniazid and ethambutol were correlated with a dramatic decrease of cell surface hydrophobicity, reflecting the removal of the outermost mycolic acid layer. Consistent with this finding, tapping mode imaging, combined with immunogold labeling, showed that the two drugs lead to the massive exposure of hydrophilic lipoarabinomannans at the surface. Ta...Continue Reading

References

Jul 14, 1995·Science·P A SielingP J Brennan
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·L DengM R McNeil
Nov 1, 1995·Antimicrobial Agents and Chemotherapy·K MikusováP J Brennan
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·A E BelangerJ M Inamine
Nov 5, 1997·Advances in Microbial Physiology·M Daffé, P Draper
Jul 17, 1998·Current Opinion in Chemical Biology·D Chatterjee
Dec 16, 1998·Frontiers in Bioscience : a Journal and Virtual Library·P Draper
Dec 28, 1999·Microbes and Infection·G R Strohmeier, M J Fenton
Sep 10, 2002·Journal of Bacteriology·Yves F Dufrêne
May 21, 2004·Nature Reviews. Microbiology·Yves F Dufrêne
Feb 16, 2006·Drug Discovery Today·Ying ZhangSteven Denkin
Mar 21, 2006·Lancet·Christopher Dye
May 18, 2006·The Journal of Biological Chemistry·Premkumar DinadayalaDean C Crick
Oct 18, 2006·Antimicrobial Agents and Chemotherapy·Christophe PossozMarcelo E Tolmasky
Oct 27, 2006·FEMS Microbiology Letters·Claire VerbelenYves F Dufrêne
Mar 10, 2007·Current Topics in Medicinal Chemistry·Patrick J Brennan, Dean C Crick

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Citations

Jun 25, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Carla SilvaLuisa Jordao
Oct 27, 2010·Nature Communications·Guillaume AndreYves F Dufrêne
Oct 7, 2008·Journal of Bacteriology·Grégory FranciusYves F Dufrêne
Aug 16, 2011·Journal of Bacteriology·Rakesh K DhimanDean C Crick
Mar 10, 2011·Nanomedicine·David AlsteensYves F Dufrêne
Mar 9, 2011·Chemical Communications : Chem Comm·Nicholas P WestRichard J Payne
May 9, 2014·Expert Opinion on Drug Delivery·Constanze LamprechtAndreas Ebner
Nov 24, 2012·Sensors and Actuators. B, Chemical·Leslie A Hiatt, David E Cliffel
Jun 15, 2011·Trends in Cell Biology·Daniel J Müller, Yves F Dufrêne
Jul 16, 2010·Trends in Microbiology·Vincent DupresYves F Dufrêne
Jun 12, 2010·Micron : the International Research and Review Journal for Microscopy·Yangzhe Wu, Anhong Zhou
Mar 7, 2009·Current Opinion in Biotechnology·Daniel J MüllerYves F Dufrêne
Mar 13, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Giovanni Longo, Sandor Kasas
Dec 21, 2010·Research in Microbiology·Antonietta La StoriaGianluigi Mauriello
Jun 8, 2012·Micron : the International Research and Review Journal for Microscopy·Loredana S DorobantuRobert E Burrell
Feb 27, 2008·The Analyst·Yves F Dufrêne
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·T P Tim CushnieAndrew J Lamb
Jun 27, 2014·Physical Chemistry Chemical Physics : PCCP·Yangzhe WuAnhong Zhou
Feb 6, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Mi LiWeijing Zhang
May 18, 2018·Future Microbiology·Parul SinghSangita Mukhopadhyay
Aug 30, 2013·Nature Methods·Yves F DufrêneDaniel J Müller
Jul 26, 2008·Nature Nanotechnology·Daniel J Müller, Yves F Dufrêne
Jul 16, 2008·Nature Reviews. Microbiology·Yves F Dufrêne
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Claire Verbelen, Yves F Dufrêne
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·David AlsteensYves F Dufrêne
May 19, 2009·Nature Chemical Biology·Daniel J MüllerYves F Dufrêne
Mar 19, 2020·Cells·Albertus ViljoenYves Dufrêne
Apr 22, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Albertus ViljoenYves F Dufrêne
Oct 5, 2017·Annals of Clinical Microbiology and Antimicrobials·Yuhui XuZhaogang Sun
Jan 25, 2020·Nature Communications·Mélanie T M HannebelleGeorg E Fantner
Jul 17, 2020·Frontiers in Microbiology·Yves F Dufrêne, Albertus Viljoen
Feb 27, 2020·MBio·Albertus ViljoenYves F Dufrêne
Apr 8, 2020·Journal of Bacteriology·Albertus ViljoenYves F Dufrêne

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