PMID: 7547474Jun 1, 1995Paper

Organization of Wolbachia pipientis in the Drosophila fertilized egg and embryo revealed by an anti-Wolbachia monoclonal antibody

Mechanisms of Development
H Kose, T L Karr

Abstract

Cytoplasmic incompatibility (CI) in Drosophila is related to the presence of Wolbachia, an intracellular microorganism found in many species of insects. In order to study the intracellular localization of Wolbachia in eggs and embryos, we have purified the bacteria from fly embryos and subsequently generated a monoclonal antibody (Mab Wol-1) specific for Wolbachia. Indirect immunofluorescence staining using Wol-1 reveals that during mitosis, Wolbachia are localized near spindle poles and centrosomes. Double label immunofluorescence experiments using anti-tubulin and anti-Wolbachia antibodies show that Wolbachia co-localize with centrosomal microtubules throughout the cell cycle. Direct interactions between the bacteria and centrosome-organized microtubules are implied from seven observations: (1) throughout the mitotic cycle, the position and movement of Wolbachia precisely mimic the behavior of the centrosome and apparently associated with centrosome-organized microtubules; (2) Wolbachia segregate equally to each spindle pole during mitosis; (3) Wolbachia do not associate with spindle microtubules during mitosis; (4) Wolbachia located in the egg cortex localize to the domains of cytoplasm organized by microtubules during blast...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1986·The Journal of Cell Biology·T L Karr, B M Alberts
Sep 1, 1973·Journal of Invertebrate Pathology·J H Yen, A R Barr
May 1, 1973·Developmental Biology·M Zalokar
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·C C Dapples, R C King
Jan 1, 1984·The Journal of Cell Biology·R M WarnS Webb
May 1, 1982·Rheumatology and Rehabilitation·D Wheatley
Jun 1, 1994·Current Biology : CB·T L Karr
Oct 28, 1994·Science·L Cooley, W E Theurkauf
Jan 1, 1959·Cold Spring Harbor Symposia on Quantitative Biology·H LAVEN
Jun 1, 1970·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·Erich Jost

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Citations

Aug 28, 2003·Microbes and Infection·Uyen TramWilliam Sullivan
Sep 17, 2008·Nature Reviews. Microbiology·John H WerrenMichael E Clark
Dec 21, 2000·Insect Molecular Biology·K BourtzisS L O'Neill
Sep 5, 2006·Applied and Environmental Microbiology·Jason L RasgonXiaoxia Ren
Sep 4, 2004·Applied and Environmental Microbiology·Zoe VenetiKostas Bourtzis
Jan 1, 1997·Annual Review of Entomology·J H Werren
Aug 21, 2008·Annual Review of Genetics·Laura R SerbusWilliam Sullivan
Feb 1, 2012·PloS One·Maria Giovanna RiparbelliGiuliano Callaini
Apr 19, 2005·Genetics·Sylvain CharlatHervé Merçot
Oct 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Natsuko KondoTakema Fukatsu
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K T Min, S Benzer
Sep 23, 2008·Current Biology : CB·Patrick M FerreeJohn H Werren
Nov 5, 1999·Annual Review of Microbiology·R StouthamerG D Hurst
Apr 24, 2015·PLoS Pathogens·Irene L G NewtonKathy B Sheehan
Jun 10, 2016·FEMS Microbiology Letters·Orlando YañezPeter Neumann
Jul 28, 2016·MicrobiologyOpen·Jose E PietriWilliam Sullivan
Jun 28, 2016·Applied and Environmental Microbiology·Steen ChristensenLaura R Serbus
Sep 26, 2020·ELife·J Dylan ShropshireSeth R Bordenstein
Dec 19, 2007·PLoS Pathogens·Laura R Serbus, William Sullivan
Nov 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Aurélien RichaudMarie-Anne Félix
Feb 22, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Denis PoinsotHervé Merçot
Jan 28, 2019·Trends in Genetics : TIG·John F BeckmannSylvain Charlat

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