Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning

Molecular Plant
Xiaolu QuShanjin Huang

Abstract

Polarized tip growth is a fundamental cellular process in many eukaryotes. In this study, we examined the dynamic restructuring of the actin cytoskeleton and its relationship to vesicle transport during pollen tip growth in Arabidopsis. We found that actin filaments originating from the apical membrane form a specialized structure consisting of longitudinally aligned actin bundles at the cortex and inner cytoplasmic filaments with a distinct distribution. Using actin-based pharmacological treatments and genetic mutants in combination with FRAP (fluorescence recovery after photobleaching) technology to visualize the transport of vesicles within the growth domain of pollen tubes, we demonstrated that cortical actin filaments facilitate tip-ward vesicle transport. We also discovered that the inner apical actin filaments prevent backward movement of vesicles, thus ensuring that sufficient vesicles accumulate at the pollen tube tip to support the rapid growth of the pollen tube. The combinatorial effect of cortical and internal apical actin filaments perfectly explains the generation of the inverted "V" cone-shaped vesicle distribution pattern at the pollen tube tip. When pollen tubes turn, apical actin filaments at the facing side ...Continue Reading

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Citations

Nov 28, 2018·Journal of Experimental Botany·Ludi WangMaurice Bosch
May 2, 2019·Nature Plants·Yanjun YuZhaosheng Kong
Feb 14, 2020·Journal of Cell Science·Wanying ZhaoShanjin Huang
Mar 17, 2020·Journal of Experimental Botany·Jingzhe Guo, Zhenbiao Yang
Feb 10, 2018·Journal of Integrative Plant Biology·Min DiaoShanjin Huang
May 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yuxiang JiangShanjin Huang
Nov 25, 2018·International Journal of Molecular Sciences·Shujuan ZhangHui Su
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Yanan Xu, Shanjin Huang
Mar 7, 2021·International Journal of Molecular Sciences·Chaoxia LuBaoshan Wang
Sep 24, 2019·Current Opinion in Cell Biology·Robert G OrrMagdalena Bezanilla
Apr 2, 2021·Plant Physiology·Maki Hayashi, Michael Palmgren
May 5, 2021·Plant Signaling & Behavior·Mohammad Foteh Ali, Tomokazu Kawashima
May 26, 2021·Current Biology : CB·Youssef ChebliAnja Geitmann
Sep 25, 2021·The Plant Journal : for Cell and Molecular Biology·Xiulin TianYan Li

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