Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signaling

Integrative Biology : Quantitative Biosciences From Nano to Macro
Sebastián L VegaPrabhas V Moghe

Abstract

Stem cell fates on biomaterials are influenced by the complex confluence of microenvironmental cues emanating from soluble growth factors, cell-to-cell contacts, and biomaterial properties. Cell-microenvironment interactions influence the cell fate by initiating a series of outside-in signaling events that traverse from the focal adhesions to the nucleus via the cytoskeleton and modulate the sub-nuclear protein organization and gene expression. Here, we report a novel imaging-based framework that highlights the spatial organization of sub-nuclear proteins, specifically the splicing factor SC-35 in the nucleoplasm, as an integrative marker to distinguish between minute differences of stem cell lineage pathways in response to stimulatory soluble factors, surface topologies, and microscale topographies. This framework involves the high resolution image acquisition of SC-35 domains and imaging-based feature extraction to obtain quantitative nuclear metrics in tandem with machine learning approaches to generate a predictive cell state classification model. The acquired SC-35 metrics led to >90% correct classification of emergent human mesenchymal stem cell (hMSC) phenotypes in populations of hMSCs exposed for merely 3 days to basal,...Continue Reading

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Citations

Feb 15, 2016·Bioorganic & Medicinal Chemistry Letters·Yajun HuangWeiping Zheng
Dec 15, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Simon GordonovMark Bathe
May 12, 2015·Free Radical Biology & Medicine·Stefania FilosaJoussef Hajek
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Nov 6, 2018·Scientific Reports·Anandika DhaliwalPrabhas V Moghe
Jul 3, 2021·Chemical Reviews·Farhad SoheilmoghaddamJustin Cooper-White
Apr 4, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sophie A LelièvreShirisha Chittiboyina

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