Organizational variation of DYZ1 repeat sequences on the human Y chromosome and its diagnostic potentials

DNA and Cell Biology
Mohammed Mahidur RahmanSher Ali

Abstract

The long arm of the human Y chromosome is flecked with various fractions of repetitive DNA. DYZ1 is one such fraction, which is organized tandemly as an array of a 3.4-kb repeat ranging from 2000-4000 copies in normal males. We have studied the organizational variation of the DYZ1 fraction on the human Y chromosome using DNA samples from CEPH family members and the random population employing the RFLP approach, fluorescence in situ hybridization (FISH), and conducted a similarity search with GenBank sequences. Typing of genomic DNA using DYZ1 as a probe showed an allele length and copy number variations even between two male siblings. Hybridization of DNA from monochromosome hybrids with this probe showed its presence on chromosome 15 in addition to the Y chromosome. Fluorescence in situ hybridization of metaphase chromosomes from an apparently normal male showed DYZ1 sequences in the proximal region of chromosome 11 in addition to the long arm of the Y chromosome. Typing of sets of semen and blood DNA samples from the same human individuals showed discernible allelic variation between the two samples, indicating tissue-specific programmed sequence modulation. DYZ1 seems to be the first probe having the unique potential to disc...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B Charlesworth
Apr 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J A Graves
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·J W Foster, J A Graves
Nov 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J A Graves
Nov 5, 1999·Science·B T Lahn, D C Page
Mar 10, 2001·Nature·C A TilfordD C Page
Mar 21, 2001·Nature Reviews. Genetics·B T LahnK Jegalian
Feb 28, 2002·Gene·Sher Ali, Seyed Ehtesham Hasnain
Apr 23, 2002·Current Biology : CB·Alan J ReddMichael F Hammer
Jun 25, 2002·Human Reproduction Update·Sherman J Silber, Sjoerd Repping
May 1, 1964·Mutation Research·H J MULLER

❮ Previous
Next ❯

Citations

May 17, 2014·PLoS Computational Biology·Nicolas AltemoseHuntington F Willard
Jan 1, 2005·Journal of Molecular and Genetic Medicine : an International Journal of Biomedical Research·Cláudia Márcia Benedetto de Carvalho, Fabrício Rodrigues Santos
Aug 26, 2010·Journal of Forensic Sciences·David R ForanJohn H Trestrail
Jan 27, 2020·Molecular Biology Reports·Hirak R DashSurajit Das
Feb 6, 2014·BMC Genetics·Sandeep Kumar YadavSher Ali

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.