Organoid culture systems for prostate epithelial and cancer tissue

Nature Protocols
Jarno DrostHans Clevers

Abstract

This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy mouse and human prostate cells (either bulk or FACS-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumor cells. Organoids derived from healthy material contain the differentiated luminal and basal cell types, whereas organoids derived from prostate cancer tissue mimic the histology of the tumor. We explain how to establish these cultures in the fully defined serum-free conditioned medium that is required to sustain organoid growth. Starting with the plating of digested tissue material, full-grown organoids can usually be obtained in ∼2 weeks. The culture protocol we describe here is currently the only one that allows the growth of both the luminal and basal prostatic epithelial lineages, as well as the growth of advanced prostate cancers. Organoids established using this protocol can be used to study many different aspects of prostate biology, including homeostasis, tumorigenesis and drug discovery.

Associated Clinical Trials

References

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Aug 20, 2005·Science·Kyung-Ah KimKazuma Tomizuka
Jan 24, 2008·The Prostate·Kenneth J PientaRobert L Vessella
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Citations

Apr 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jung Wook ParkOwen N Witte
Sep 7, 2016·Cancers·Benjamin LuTerence J Van Raay
Jul 30, 2016·BMC Medicine·Tommaso A DraganiJack A Schalken
Mar 28, 2017·Trends in Molecular Medicine·Devanjali DuttaHans Clevers
May 24, 2017·Nature Reviews. Urology·Shangqian WangYu Chen
Mar 21, 2017·Molecular and Cellular Endocrinology·Alastair H DaviesAmina Zoubeidi
Apr 7, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Zuzana KahounováKarel Souček
Feb 22, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ninouk Akkerman, Libert H K Defize
Jan 20, 2018·Advanced Healthcare Materials·Darren RodenhizerAlison P McGuigan
Feb 23, 2018·The Journal of Cell Biology·Florence Broders-BondonEmmanuel Farge
Mar 16, 2017·Development·Jarno Drost, Hans Clevers
May 18, 2018·Stem Cells·Garima KaushikSurinder K Batra
Aug 18, 2018·Molecular Oncology·Margaret M CenteneraWayne D Tilley
Dec 21, 2018·Omics : a Journal of Integrative Biology·Kevin DzoboM Iqbal Parker
Jul 7, 2017·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Eugenia GirdaLloyd H Smith
Nov 30, 2018·The Journal of Clinical Investigation·Yufeng DingJun Qin
May 31, 2018·Cold Spring Harbor Perspectives in Medicine·Loredana PucaHimisha Beltran
Feb 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jasper MullendersHans C Clevers
Mar 8, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tomoya MiyoshiRyuji Morizane
May 17, 2019·The Journal of Cell Biology·Dawid G NowakLloyd C Trotman
Jan 26, 2018·Journal of the Royal Society, Interface·Aleksandra KarolakKatarzyna A Rejniak
Feb 26, 2019·EMBO Reports·Stéphanie TorrinoStéphan Clavel
Aug 1, 2019·Biology Open·Manja OmerzuMadelon M Maurice
Jul 10, 2019·The EMBO Journal·Margit BleijsJarno Drost
Feb 23, 2020·The Prostate·Anna M GleaveYuzhuo Wang

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Methods Mentioned

BETA
biopsy
Dissection
FACS
biopsies

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