Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases

Cell Death & Disease
Ludovica GrassiDésirée Bonci

Abstract

The pressure towards innovation and creation of new model systems in regenerative medicine and cancer research has fostered the development of novel potential therapeutic applications. Kidney injuries provoke a high request of organ transplants making it the most demanding system in the field of regenerative medicine. Furthermore, renal cancer frequently threaten patients' life and aggressive forms still remain difficult to treat. Ethical issues related to the use of embryonic stem cells, has fueled research on adult, patient-specific pluripotent stem cells as a model for discovery and therapeutic development, but to date, normal and cancerous renal experimental models are lacking. Several research groups are focusing on the development of organoid cultures. Since organoids mimic the original tissue architecture in vitro, they represent an excellent model for tissue engineering studies and cancer therapy testing. We established normal and tumor renal cell carcinoma organoids previously maintained in a heterogeneous multi-clone stem cell-like enriching medium. Starting from adult normal kidney specimens, we were able to isolate and propagate organoid 3D-structures composed of both differentiated and undifferentiated cells while ...Continue Reading

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Methods Mentioned

BETA
mechanical dissociation
RNAseq
PCR
RNA-Seq
confocal microscopy
protein array
FACS
xenograft

Software Mentioned

Kallisto
R
tidyverse
plyr
ggbeeswarm
ggsignif
Omics
exactRankTests

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