Organotin-induced apoptosis as observed in vitro is not relevant for induction of thymus atrophy at antiproliferative doses

Toxicology and Applied Pharmacology
Alessandra GennariR H Pieters

Abstract

The organotin compounds di-n-butyltin dichloride (DBTC) and tri-n-butyltin chloride (TBTC) selectively cause thymus atrophy. Previously, DBTC and TBTC were shown to inhibit proliferation of immature thymocytes, but other studies demonstrated that TBTC but not DBTC increased apoptosis in vitro and also in vivo. In this study, we examined whether apoptosis is increased in vitro by DBTC and TBTC at various concentrations and periods of incubation and whether apoptosis is involved in the induction of thymus atrophy at selective antiproliferative doses. In vitro, DBTC or TBTC at a concentration of 3 microM significantly increased DNA fragmentation in freshly isolated rat thymocytes after preincubation for 10 min followed by a 22-hr culture period. After continuous exposure for 22 hr, apoptosis was observed to be optimum at 1 microM TBTC and 0.3 microM DBTC and lower or absent at higher concentrations. Apparently, apoptosis induced by organotins in vitro depends on both duration and concentration of exposure. Selective antiproliferative doses of DBTC nor TBTC increased apoptosis on day 1 or 2 after single oral exposure. In contrast, the corticosteroid dexamethasone caused a depletion of both small and large thymocytes and a marked in...Continue Reading

Citations

Jul 23, 2002·Journal of Toxicology and Environmental Health. Part a·Valérie Lavastre, Denis Girard
Sep 2, 2009·The Journal of Veterinary Medical Science·Shunji UenoMasayasu Sugiyama
Nov 11, 1999·Human & Experimental Toxicology·A De Santiago, M Aguilar-Santelises
Aug 26, 2017·Journal of Applied Toxicology : JAT·Shyretha BrownMargaret Whalen
Jan 1, 2014·Journal of Applied Toxicology : JAT·Ahmed M Osman, Henk van Loveren
Sep 8, 2018·Journal of Cellular Biochemistry·Lei-Lei YangShen Yin
Aug 2, 2007·Journal of Applied Toxicology : JAT·Biplob KochAnupam Chatterjee
Oct 27, 2009·Apoptosis : an International Journal on Programmed Cell Death·Geneviève Dupéré-MinierJacques Bernier
Mar 11, 2006·Environmental Toxicology·Hui-Gang LiuLi-Hong Xu
Oct 30, 2008·Journal of Immunotoxicology·Kirsten A BakenHenk van Loveren
Aug 15, 2009·Journal of Immunotoxicology·Kenichi TomiyamaYasuaki Arakawa
May 18, 2016·Journal of Applied Toxicology : JAT·Shyretha BrownMargaret M Whalen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis