Organotypic culture, a powerful model for studying rat and mouse fetal testis development

Cell and Tissue Research
Gabriel LiveraRené Habert

Abstract

The key role of the fetal testis in the masculinization of genital organs has been known for a long time. More recently, the observed increases in male reproductive disorders has been postulated to be the result of changes in fetal and neonatal testis development in response to increasing environmental pollution. However, few tools are available for studying fetal testis development and the effects of physiological or toxic substances. We have developed an organ culture system in which rat fetal testis is grown on a filter floating on a synthetic medium containing no serum, hormones or biological factors. In this study, we have compared the long-term morpho-functional development of the various testicular cell types in this system with that observed in vivo and have extended this system to the mouse. Rat Leydig, Sertoli and germ cells and macrophages develop normally over a period of 1-2 weeks in this system. Fewer cells are produced than in vivo but the level of differentiated function is similar. Germ cells, which are difficult to culture in vitro, resume mitosis after a quiescent period, at the same time as in vivo. Similar results have been obtained with mouse fetuses, except that Leydig cells dedifferentiate in vitro if th...Continue Reading

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Citations

Jul 31, 2012·Cell and Tissue Research·Camila DoresIna Dobrinski
Jul 20, 2012·Human Reproduction·P TartarinP Froment
Jul 14, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Abdelali LehraikiChristine Levacher
Feb 11, 2010·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·T Rhen, A Schroeder
Jan 13, 2010·Environmental Health Perspectives·Gaëlle AngenardGabriel Livera
Sep 27, 2013·PLoS Genetics·Benoit SouquetGabriel Livera
Jan 14, 2010·Folia Histochemica Et Cytobiologica·René HabertVirginie Rouiller-Fabre
Jan 14, 2010·Folia Histochemica Et Cytobiologica·Virginie Rouiller-FabreRené Habert
Feb 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·René HabertGabriel Livera
May 6, 2014·Annales d'endocrinologie·Virginie Rouiller-FabreGabriel Livera
Jan 24, 2009·Environmental Health Perspectives·Romain LambrotVirginie Rouiller-Fabre
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tony DeFalcoBlanche Capel
Apr 10, 2012·Toxicology and Applied Pharmacology·V MuczynskiV Rouiller-Fabre
Jan 26, 2011·Reproductive Toxicology·Gaëlle AngenardVirginie Rouiller-Fabre
Mar 5, 2016·Journal of Applied Toxicology : JAT·Anne-Marie SaillenfaitDelphine Moison
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Sep 15, 2006·Gynécologie, obstétrique & fertilité·R HabertC Levacher
Jun 1, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Abdelali LehraikiChristine Levacher
May 23, 2015·Frontiers in Endocrinology·Virginie Rouiller-FabreGabriel Livera
Jun 15, 2010·Fertility and Sterility·Mara CurabaJacques Donnez
Mar 30, 2010·Developmental Biology·Stéphanie G MorenoRené Habert
May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jinhwan LimUlrike Luderer
Jul 17, 2016·Journal of Reproductive Immunology·Sudhanshu Bhushan, Andreas Meinhardt
Nov 13, 2018·Endocrinology·Halima AlbalushiJan-Bernd Stukenborg

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