Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination

Methods in Molecular Biology
Amin SherafatAkiko Nishiyama

Abstract

Oligodendrocyte development and myelination are processes in the central nervous system that are regulated by cell intrinsic and extrinsic mechanisms. Organotypic slice cultures provide a simple method for studying factors that affect oligodendrocyte proliferation, differentiation, and myelination in the context of the local cellular environment. Here we show that major glial cell types and neurons are preserved in slice cultures from postnatal mouse forebrain, and their morphological characteristics are retained. We further demonstrate that cellular processes requiring interactions with neighboring cells such as myelination can proceed in slice culture.

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

Journal of Visualized Experiments : JoVE
Robert A HillAkiko Nishiyama
Seikagaku. The Journal of Japanese Biochemical Society
Jin Nakahara, Hiroaki Asou
Sheng li ke xue jin zhan [Progress in physiology]
Peng-Hui Chen, Wen-Qin Cai
© 2022 Meta ULC. All rights reserved