Oridonin inhibits cell growth by induction of apoptosis on human hepatocelluar carcinoma BEL-7402 cells

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Jun-Feng ZhangGui-Hua Chen

Abstract

The present study was undertaken to investigate the mechanisms of oridonin induced apoptosis on human hepatocellular carcinoma BEL-7402 cells. BEL-7402 cells in culture medium in vitro were given different concentrations of oridonin. Cell proliferation was measured by MTT assay and [(3)H]-thymidine uptake, cell apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by Hoechst 33258 staining and DNA fragmentation. Caspase-3 as well as Bcl-2 and Bax expressions were detected by Western blotting. The results revealed that oridonin could inhibit the growth of BEL-7402 cells and cause apoptosis significantly, the suppression was both in time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed very clearly by Hoechst 33258 staining as well as DNA fragmentation analysis after the cells exposed to oridonin for 60h; Western blotting showed cleavage of the caspase-3 zymogen protein (32-kDa) with the appearance of its 20-kDa subunit; Along with the apoptotic process Bcl-2 expression was down-regulated and Bax expression up-regulated concurrently observed by Western blotting. We therefore conclude that oridonin can inhibit cell growth by induction of apoptosis in ...Continue Reading

Citations

Oct 26, 2007·Acta Pharmacologica Sinica·Hong-zhen HuHai-tao Zeng
Dec 23, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Li-Hong YeJian-Song He
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Jan 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuanyuan ZhangHuagang Sheng
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