Oridonin inhibits metastasis of human ovarian cancer cells by suppressing the mTOR pathway

Archives of Medical Science : AMS
Ye Wang, Zhiling Zhu

Abstract

Oridonin, which is isolated from the Chinese herb Rabdosia rubescens, has been reported to exhibit an anti-tumorous effect on different cancers. In this study, we investigated the molecular mechanism by which oridonin suppresses human ovarian cancer. The inhibition of oridonin on cell proliferation was assessed by CCK8 assay. Cell cycle and apoptosis were analyzed by flow cytometry, staining with propidium iodide (PI) or annexin-V/PI respectively. The metastasis rate was evaluated using a transwell migration assay. The expression of metastasis-associated genes and mTOR pathway related genes were detected by western blot. We demonstrated that oridonin suppressed the proliferation and blocked the cell cycle in G1/S phage and induced apoptosis in SKOV3 and A2780 cells (p < 0.01). We further found that the mTOR signaling pathway was suppressed by the treatment with oridonin, and the activation of the mTOR pathway attenuated the anti-tumorous effect of oridonin in human ovarian cancer cells, suggesting that the mTOR pathway was involved in the anti-tumorous process of oridonin. Additionally, the activation of the mTOR pathway by an exogenous activator reduced the expression level of FOXP3 (p < 0.01), thus providing evidence that FOX...Continue Reading

Citations

Nov 22, 2019·Pharmaceutical Biology·Jing LiuYing Li
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kailong WangHaiyang Yu
Feb 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nurul Akmaryanti AbdullahNoraina Muhamad Zakuan

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Methods Mentioned

BETA
transfection
protein assay
electrophoresis
flow cytometry

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