Orientation and conformation of osteocalcin adsorbed onto calcium phosphate and silica surfaces

Biointerphases
Luisa A ScudellerDavid G Castner

Abstract

Adsorption isotherms, circular dichroism (CD) spectroscopy, x-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were used to investigate the adsorption of human osteocalcin (hOC) and decarboxylated (i.e., Gla converted back to Glu) hOC (dhOC) onto various calcium phosphate surfaces as well as silica surfaces. The adsorption isotherms and XPS nitrogen signals were used to track the amount of adsorbed hOC and dhOC. The intensities of key ToF-SIMS amino acid fragments were used to assess changes in the structure of adsorbed hOC and dhOC. CD spectra were used to investigate the secondary structure of OC. The largest differences were observed when the proteins were adsorbed onto silica versus calcium phosphate surfaces. Similar amounts (3-4 at. % N) of hOC and dhOC were adsorbed onto the silica surface. Higher amounts of hOC and dhOC were adsorbed on all the calcium phosphate surfaces. The ToF-SIMS data showed that the intensity of the Cys amino acid fragment, normalized to intensity of all amino acid fragments, was significantly higher (∼×10) when the proteins were adsorbed onto silica. Since in the native OC structure the cysteines are located in the center of three α-helices, this...Continue Reading

References

Oct 15, 1992·The New England Journal of Medicine·F L CoeJ R Asplin
Jul 1, 1989·Physiological Reviews·P V HauschkaC M Gundberg
Mar 1, 1985·Calcified Tissue International·R Z LeGeros
May 11, 1982·Biochemistry·P V Hauschka, S A Carr
Sep 1, 1995·European Journal of Biochemistry·R A AtkinsonR J Williams
Dec 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J R Cairns, P A Price
Jun 1, 1994·Disease-a-month : DM·D J McCarty
Aug 1, 1996·Nature·P DucyG Karsenty
Jan 1, 1996·Connective Tissue Research·P G Robey
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T L ClemensC M Gundberg
Jul 21, 2000·Annals of Clinical Biochemistry·A J LeeR Eastell
May 9, 2001·Biochimica Et Biophysica Acta·T L DowdC M Gundberg
Jan 9, 2003·The Journal of Biological Chemistry·Satoru K NishimotoRichard W Kriwacki
Feb 22, 2003·Science·Trevor Douglas
Oct 31, 2003·Nature·Quyen Q HoangDaniel S C Yang
Oct 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Sarah L BoothDouglas P Kiel
Apr 2, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hua WangShaoyi Jiang
Aug 19, 2007·Cell·Na Kyung LeeGerard Karsenty
Dec 18, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Willi Jahnen-DechentMarc D McKee
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mathieu FerronPatricia Ducy
Mar 28, 2008·Nutrition Reviews·George Wolf
Feb 22, 2011·Cell·Franck OuryGerard Karsenty
Apr 9, 2011·Nature·Sonya M Schuh-Huerta, Renee A Reijo Pera
Jul 16, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Anna IdelevichEfrat Monsonego-Ornan
Apr 21, 2012·Advances in Nutrition·Caren M GundbergSarah L Booth
Aug 16, 2012·Biointerphases·Daniel J Graham, David G Castner
Nov 14, 2012·Nature Reviews. Endocrinology·Sarah L BoothCaren Gundberg
Jun 4, 2013·Physical Chemistry Chemical Physics : PCCP·Tobias Weidner, David G Castner
Jun 6, 2013·International Journal of Endocrinology·Aurora PattiRanuccio Nuti
Mar 16, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Rami N FosterDavid G Castner

❮ Previous
Next ❯

Citations

Jul 1, 2017·Biointerphases·Tobias Weidner
Jul 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kyungsoo ShinFrancesca M Marassi
Apr 2, 2021·Molecular Systems Design & Engineering·Sarah Alamdari, Jim Pfaendtner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B
Joe E BaioDavid G Castner
Journal of Peptide Science : an Official Publication of the European Peptide Society
Layal KaramNour-Eddine Chihib
© 2022 Meta ULC. All rights reserved