PMID: 6972230May 13, 1981Paper

Orientation of intrinsic proteins in photosynthetic membranes. Polarized infrared spectroscopy of chloroplasts and chromatophores

Biochimica Et Biophysica Acta
E Nabedryk, J Breton

Abstract

In order to estimate the degree of orientation of the alpha-helices of intrinsic proteins in photosynthetic membranes, polarized infrared spectroscopy has been used to measure the dichroism of the amide I and amide II absorption bands of air-dried oriented samples of purple membranes, chloroplasts and chromatophores from Rhodopseudomonas sphaeroides. Using purple membrane, in which the orientation of the alpha-helices is precisely known (Henderson, R. (1977) Annu. Rev. Biophys. Bioeng. 6, 87-109), as a standard to calibrate our measurements and estimating the mosaic spread (extent of orientation) of the membranes from linear dichroism measurements performed in the visible spectral range, it is concluded that in photosynthetic membranes, the alpha-helices of intrinsic proteins are tilted at less than 40 degrees with respect to the normal to the plane of the membrane.

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Citations

Jan 1, 1994·Journal of Protein Chemistry·L LinsJ M Ruysschaert
Jan 1, 1999·Chemistry and Physics of Lipids·L Silvestro, P H Axelsen
Dec 1, 1994·Photochemistry and Photobiology·J D Olsen, C N Hunter
Apr 25, 2000·Biophysical Journal·D MarshF J Schmitt
Feb 13, 2001·Biophysical Journal·D Marsh, T Páli
Jan 15, 1992·European Journal of Biochemistry·G VandenbusscheJ M Ruysschaert
Jun 22, 2017·Journal of the American Chemical Society·Shin Nakamura, Takumi Noguchi
Feb 4, 2019·Photosynthesis Research·Paul MathisAndré Verméglio
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Jul 7, 1999·Biochimica Et Biophysica Acta·E GoormaghtighJ M Ruysschaert
Sep 7, 1983·Biochimica Et Biophysica Acta·H VogelJ Stümpel
Jan 10, 1998·Biochemistry·J F HuntD M Engelman

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