Abstract
The orientation of alpha-helices or beta-strands, e.g., in membranes, can be determined from EPR order parameters of (2,2,6,6-tetramethyl-piperidine-1-oxy-4-amino-4-carboxylic acid) TOAC amino-acid spin labels incorporated in the polypeptide backbone. This requires knowledge of the inclination of the nitroxide axes, relative to the alpha-helix or beta-strand axis. Crystal structures of TOAC-containing peptides are used to derive the spin-label orientation relative to refined alpha-poly-l-alanine and beta-poly-l-alanine structures. The spin-label z-axes of the two mirror-image TOAC twist-boat conformers are inclined at 13+/-2 degrees and 65+/-3 degrees , respectively, to the alpha-helix axis, or at 25+/-3 degrees and 32+/-3 degrees to the beta-strand axis.
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Citations
Aug 11, 2010·Biochemistry·Ned Van EpsGarland R Marshall
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Nov 13, 2016·Bioorganic Chemistry·Luis Gustavo Deus TeixeiraClovis R Nakaie
Jun 26, 2007·Chemistry & Biodiversity·Derek MarshClaudio Toniolo
Dec 5, 2009·Applied Magnetic Resonance·Derek Marsh
Sep 4, 2009·Biopolymers·Renata F F VieiraClóvis R Nakaie
Sep 6, 2011·Physical Chemistry Chemical Physics : PCCP·Ya-Wen Huang, Yun-Wei Chiang
Sep 8, 2011·Angewandte Chemie·Sven StollerUlf Diederichsen
Jan 26, 2018·Applied Magnetic Resonance·Derek Marsh
Mar 1, 2012·Biophysics Reviews·Shirley SchreierClóvis R Nakaie
Nov 7, 2019·The Journal of Physical Chemistry. B·Peter D MartinStefan Stoll