Oriented assembly of invisible probes: towards single mRNA imaging in living cells

Chemical Science
Xiang-Ling LiHong-Yuan Chen

Abstract

Due to the complexity of biological systems and the ultralow concentration of analytes, improving the signal-to-noise ratio and lowering the limit of detection to allow highly sensitive detection is key to biomolecule analysis, especially intracellular analysis. Here, we present a method for highly sensitive imaging of mRNA in living cells by using novel invisible oriented probes to construct a turn-on signal generation mechanism from zero background. Two DNA probes (S1 and S2) are asymmetrically modified on two small gold nanoparticles (AuNPs) with a diameter of 20 nm. The hybridization of the two DNA probes with a single target mRNA leads to the formation of an AuNP dimer which shows a prominent plasmonic coupling effect. It generates a strong scattering signal from zero-background under a dark-field spectral analysis system. The unique design of the oriented assembly dimer has the ability to easily discriminate the target signal from the inherent cellular background noise in intracellular detection, thus making this approach a valuable technique for imaging single survivin mRNA and monitoring the distribution of survivin mRNA in tumor cells.

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Citations

Jul 1, 2018·Chemistry : a European Journal·Jing XiaMing-Yong Han
Jul 15, 2016·Chemical Communications : Chem Comm·Guang-Sheng QianHong-Yuan Chen

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Methods Mentioned

BETA
surface plasmon resonance
biosensor
plasmon
atomic
transmission electron microscopy
AFM
dynamic light scattering
transfection
confocal microscopy
flow cytometry

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