Oriented growth of Blastocladiella emersonii in gradients of ionophores and inhibitors.

Journal of Bacteriology
R L Harold, F M Harold

Abstract

To investigate whether ion currents help to localize growth and development of Blastocladiella emersonii, we grew the organisms in gradients of various ionophores and inhibitors. Gradients were generated by placing into the culture fine glass fibers coated with insoluble inhibitors; in some cases, inhibitors were adsorbed onto beads of ion-exchange resin. Organisms growing in many of these gradients exhibited a striking tendency for the thalli to grow toward the fiber. This proved to be misleading; the cells grew not toward the source of the ionophore but into the unoccupied zone of inhibition adjacent to the fiber. Fibers coated with gramicidin-D induced marked effects on the growth of the rhizoids, which were greatly enlarged and grew toward and onto the fiber. None of the other inhibitors produced such effects, except for beads coated with the proton conductors tetrachlorosalicylanilide and compound 1799. The results suggest that orientation of rhizoid growth results from enhancement of proton flux across the plasma membrane. Growth of the rhizoids was also strongly oriented by gradients of inorganic phosphate and an amino acid mixture; gradients of glucose, K+, Ca2+, and glutamate were ineffective. We propose that a major p...Continue Reading

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·L F Jaffe, R Nuccitelli
Jan 1, 1977·Annual Review of Microbiology·F M Harold
Sep 21, 1978·Biochimica Et Biophysica Acta·L C Davidse, W Flach
Dec 18, 1978·Biochimica Et Biophysica Acta·P A Friedman, E G Platzer
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Oct 15, 1976·Developmental Biology·H B Peng, L F Jaffe
Mar 22, 1976·Biochemical and Biophysical Research Communications·J HoebekeM De Brabander
Nov 1, 1974·The Journal of Cell Biology·L F Jaffe, R Nuccitelli
Feb 1, 1980·Journal of Bacteriology·J Van Brunt, F M Harold
Jan 4, 1980·Science·K R Robinson, R Cone

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Citations

Jan 1, 1986·The Journal of Membrane Biology·J H CaldwellF M Harold
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw
Jun 1, 1986·Microbiological Reviews·G T Cole
Sep 1, 1982·Developmental Biology·W K Gottschalk, D R Sonneborn
Jul 22, 2018·Fungal Genetics and Biology : FG & B·André L Gomes-VieiraJosé Roberto Meyer-Fernandes

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