Oriented nanofibrous silk as a natural scaffold for ocular epithelial regeneration

Journal of Biomaterials Science. Polymer Edition
Esmaeil BiazarSara Tavakolifard

Abstract

The aim of this study was to develop nanofibrous silk substrates for limbal stem cell expansion that can serve as a potential alternative substrate to replace human amniotic membrane. The human limbal stem cell was used to evaluate the biocompatibility of substrates (random and oriented nanofibrous mats, and human amniotic membrane) based on their phenotypic profile, viability, proliferation, and attachment ability. Biocompatibility results indicated that all substrates were highly biocompatible, as limbal stem cells could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the human limbal stem cells were firmly anchored to the substrates and were able to retain a normal corneal stem cell phenotype. Microscopic analyses illustrated that cells infiltrated the nanofibers and successfully formed a three-dimensional corneal epithelium, which was viable for 15 days. Immunocytochemistry and real-time PCR results revealed no change in the expression profile of limbal stem cells grown on nanofibrous substrates when compared to those grown on human amniotic membrane. In addition, electrospun nanofibrous silk substrates especially oriented mat provides not only a milieu supporting limbal stem ce...Continue Reading

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Citations

Feb 3, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Mohammad KhaledianEsmaeil Biazar
Dec 18, 2015·Journal of Biomaterials Science. Polymer Edition·Saeed Heidari KeshelEsmaeil Biazar
Apr 20, 2018·Journal of Medical Engineering & Technology·Esmaeil BiazarDariush Biazar
Oct 13, 2017·Biomedical Materials·Zhi ChenGordon G Wallace
Jun 11, 2017·Journal of Tissue Engineering and Regenerative Medicine·Emily A GosselinDavid L Kaplan
Jan 18, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Maryam EbrahimiSaeed Heidari-Keshel
Nov 7, 2018·Pharmaceutical Research·Simon H TranF Philipp Seib
Aug 5, 2017·Materials·Bin Kong, Shengli Mi
Nov 29, 2020·Tissue & Cell·Hamed NosratiMohammad Khodaei
Mar 6, 2021·Biomaterials Science·Himadri Shekhar RoyDeepa Ghosh
May 26, 2021·Regenerative Medicine·Farzaneh AavaniAbolfazl Nazbar
Aug 28, 2021·Nanomaterials·Rahul SharmaNikhlesh K Singh

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Methods Mentioned

BETA
biopsy
PCR
flow cytometry

Software Mentioned

Flowing

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