Oriented samples: a tool for determining the membrane topology and the mechanism of action of cationic antimicrobial peptides by solid-state NMR

Biophysics Reviews
Matthieu Fillion, Michèle Auger

Abstract

Overuse and misuse of antibiotics have led bacteria to acquire several mechanisms of resistance. In response to this, researchers have identified natural antimicrobial peptides as promising candidates to fight against multiresistant bacteria. However, their mode of action is still unclear, and a better understanding of the mode of action of these peptides is of primary importance to develop new peptides displaying high antibacterial activity and low hemolytic activity. One of the main features that defines the mechanism of action is the membrane topology of the peptide. Among the spectroscopic techniques, solid-state NMR is the technique of choice for determining the location of the peptide within the membrane. It can be achieved by performing experiments with oriented samples. In the literature, the two most common types of oriented samples are bicelles and phospholipids mechanically oriented between glass plates. The mode of perturbation of the membrane-active peptide can be studied by phosphorus-31 and deuterium NMR. On the other hand, several experiments such as nitrogen-15 and fluorine solid-state NMR, that require labeled peptides, can give valuable information on the membrane topology of the peptide. The combination of t...Continue Reading

References

Mar 21, 1983·Biochimica Et Biophysica Acta·J H Davis
Feb 1, 1980·Quarterly Reviews of Biophysics·J Seelig, A Seelig
Aug 19, 1994·Journal of Molecular Biology·R SmithA Makriyannis
Mar 6, 1998·Trends in Biotechnology·R E Hancock, R Lehrer
May 29, 1999·Antimicrobial Agents and Chemotherapy·R E Hancock, D S Chapple
Feb 13, 2001·Biophysical Journal·J A WhilesR R Vold
Apr 20, 2002·Biophysical Journal·Kevin J HallockAyyalusamy Ramamoorthy
Sep 26, 2002·Journal of the American Chemical Society·Chibing TanGyoujin Cho
Feb 25, 2003·Chemistry and Physics of Lipids·Isabelle MarcotteFrances Separovic
Sep 19, 2003·European Biophysics Journal : EBJ·M S BallaF Separovic
Dec 9, 2003·IUBMB Life·Alison Drechsler, Frances Separovic
Jul 9, 2004·Biophysical Journal·Orsolya TokeJacob Schaefer
Nov 3, 2004·Biochimica Et Biophysica Acta·Burkhard BechingerPhilippe Bertani
Dec 4, 2004·Nature Medicine·Stuart B Levy, Bonnie Marshall
Jan 1, 2005·Biophysical Journal·Mohamed N TribaPhilippe F Devaux
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Jun 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Thad A HarrounJohn Katsaras
Apr 6, 2006·Chemistry and Physics of Lipids·Mojca BesenicarGregor Anderluh
Jun 7, 2006·Molecular Membrane Biology·A James MasonBurkhard Bechinger
Jun 8, 2006·Biochimica Et Biophysica Acta·David I ChanHans J Vogel
Jul 19, 2006·Clinical Microbiology Reviews·Håvard JenssenRobert E W Hancock
Jan 25, 2007·Journal of the American Chemical Society·Sergey V DvinskikhAyyalusamy Ramamoorthy
Oct 20, 2007·Nature Protocols·Anna A De Angelis, Stanley J Opella
Dec 25, 2007·Journal of Magnetic Resonance·Stephan L GrageAnne S Ulrich
Dec 25, 2007·Journal of Magnetic Resonance·Ulrich H N DürrAnne S Ulrich
Jul 19, 2008·Science·Gary Taubes

❮ Previous
Next ❯

Citations

Apr 2, 2021·Biochimica Et Biophysica Acta. Biomembranes·Dror E WarschawskiIsabelle Marcotte
Feb 4, 2019·Chemistry and Physics of Lipids·Evgeniy S SalnikovBurkhard Bechinger
May 7, 2020·Biochimica Et Biophysica Acta. Biomembranes·Sang Ho ParkStanley J Opella

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.