Oriented Schwann cell growth on micropatterned biodegradable polymer substrates

Biomaterials
C MillerS Mallapragada

Abstract

This paper investigates the influence of substrate-mediated chemical and physical guidance on the growth and alignment of Schwann cells in vitro. Novel techniques were developed to fabricate microgrooves with adsorbed proteins on biodegradable polymer substrates made of poly(D,L-lactic acid). Compression molding and solvent-casting were used to transfer micropatterns from quartz and silicon substrates onto biodegradable polymer films. Laminin was selectively adsorbed onto the grooves and rat sciatic Schwann cells were seeded on the substrates. Laminin was found to improve adhesion of Schwann cells on the substrates. The microgrooves were found to cause the Schwann cells to align along the direction of the grooves. The groove width influenced Schwann cell alignment the most, while groove depth did not seem to play a significant role. The degradation of the grooves in the solvent cast films was much slower than those in the compression-molded films, making them the preferred substrates for Schwann cell culture.

References

Feb 1, 1983·Developmental Biology·D C TurnerM Chiquet
Apr 1, 1995·Neuropathology and Applied Neurobiology·I R GriffithsP Montague
Jan 1, 1995·Nature Medicine·J L RutkowskiG I Tennekoon
May 20, 1998·Trends in Biotechnology·C A Heath, G E Rutkowski
Jun 17, 1998·Biotechnology Progress·H C Tai, H M Buettner

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Citations

Feb 4, 2006·Annals of Biomedical Engineering·Philip P LeDucRobert M Bellin
Jun 15, 2007·Biomedical Microdevices·Shan-hui HsuChien-Hsiang Su
Dec 25, 2009·Biomedical Microdevices·Anuj A PatelSanjay Kumar
Sep 30, 2010·Journal of Materials Science. Materials in Medicine·Mingzhu SunSandra Downes
Apr 11, 2013·Journal of Materials Science. Materials in Medicine·S A MobasseriS Downes
Feb 14, 2004·Biomaterials·C L A-M Carmen Lia A-M Vleggeert-LankampR T W M Ralph T W M Thomeer
Feb 14, 2004·Biomaterials·J B Jennifer B RecknorS K Surya K Mallapragada
Apr 16, 2003·Biomaterials·Giovanni VozziSangeeta Bhatia
Feb 9, 2013·Chemical Reviews·Akon HiguchiAkihiro Umezawa
Feb 15, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tiffany Houchin-RayLonnie D Shea
Sep 26, 2002·Artificial Organs·Hiroyoshi KawakamiSunao Kubota
May 13, 2003·Tissue Engineering·Richard G PearsonKevin M Shakesheff
Aug 13, 2008·Tissue Engineering. Part a·Alejandro J AlmarzaSteven D Abramowitch
Jan 7, 2010·Tissue Engineering. Part C, Methods·Wai Yee YeongLay Poh Tan
Aug 9, 2012·Tissue Engineering. Part C, Methods·Se Heang OhJin Ho Lee
Jun 17, 2003·Journal of Biomaterials Science. Polymer Edition·Shota KanezakiSunao Kubota
Nov 27, 2009·Journal of Biomedical Science·Anuradha SubramanianSwaminathan Sethuraman
Nov 3, 2010·International Journal of Nanomedicine·Esmaeil BiazarZiba Roviemiab
May 24, 2011·Annual Review of Biomedical Engineering·Philip R LeDucJohn P Wikswo
Nov 7, 2008·Journal of Materials Science. Materials in Medicine·A PierucciA L R de Oliveira
Oct 28, 2014·ACS Applied Materials & Interfaces·Aleesha M McCormickNic D Leipzig
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Karolina ChwalekCarsten Werner
Sep 16, 2015·Journal of Bioscience and Bioengineering·Anup D SharmaDonald S Sakaguchi
Apr 9, 2015·ACS Applied Materials & Interfaces·Yueming WangWenlong Ding
Aug 1, 2009·Advanced Drug Delivery Reviews·Haoqing CaoSing Yian Chew
May 19, 2009·Biomaterials·Isaac P ClementsRavi V Bellamkonda
Aug 12, 2008·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E MartínezJ Samitier
Jul 24, 2007·Journal of Neuroscience Methods·Jianming Li, Riyi Shi
Jun 21, 2005·Annals of the New York Academy of Sciences·Jennifer B RecknorS K Mallapragada
Aug 10, 2005·Journal of Biomedical Materials Research. Part a·Jie CaiGeorge M Smith
Apr 4, 2009·Cell Motility and the Cytoskeleton·Douglas W HamiltonDonald M Brunette
Jul 16, 2003·Nursing in Critical Care·Julie Scholes
Jan 11, 2008·Journal of Biomedical Materials Research. Part a·Jianming LiRiyi Shi
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·Yvonne StarkThomas Scheper
Oct 25, 2007·Journal of Biomedical Materials Research. Part a·A Tezcaner, D Hicks
Dec 24, 2005·Macromolecular Bioscience·Gianluca Ciardelli, Valeria Chiono
Jan 31, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Uta ReichGünter Reuter

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