Origin and significance of urinary N-acetyl-beta, D-glucosaminidase (NAG) in renal patients with proteinuria

Clinica Chimica Acta; International Journal of Clinical Chemistry
M G CostiganA Shenkin

Abstract

In patients with proteinuria, indices of tubular damage are unreliable since filtered plasma enzymes could contribute to tubular enzymuria. Previous work has suggested the existence of various forms of the 'A' isoenzyme of N-acetyl-beta, D-glucosaminidase (NAG), one of which could be kidney specific and thus a useful marker of renal tubular damage. By using fast protein liquid chromatography, two forms of the 'A' isoenzyme, 'A1' and 'A2' were separated in human urine, plasma and kidney tissue. The isoenzyme profile in pathological urine resembled that seen in kidney tissue, the 'A2' isoenzyme predominating. The ratio A2/A1 in the urine of renal patients was significantly greater than in the plasma of renal patients, end-stage renal failure patients and healthy volunteers. There was no difference in the plasma ratios of the three groups studied. The clearances of total NAG, 'A1' and 'A2' isoenzymes were all greater than that of the lower molecular weight protein transferrin. This indicates that the origin of urinary NAG in patients with proteinuria is from the kidney itself. Thus, analysis of urinary NAG and its isoenzymes may be of benefit as an early predictor of renal tubular damage and may also be useful as a non-invasive in...Continue Reading

References

Dec 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·D Maruhn
Jan 1, 1991·The International Journal of Biochemistry·H A BeukesR J Naude
Aug 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Sri KrishnaA S Kanagasabapathy
Dec 8, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·B Hultberg
Feb 1, 1994·Kidney International·J D Williams, G A Coles

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Citations

Feb 6, 2013·Current Opinion in Nephrology and Hypertension·Nimrit Goraya, Donald E Wesson
Jan 1, 2010·Journal of Veterinary Internal Medicine·P M Y SmetsS Daminet
Nov 3, 2010·Journal of Veterinary Internal Medicine·B MaddensE Meyer
Apr 23, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Jesús HermidaJ Carlos Tutor
Feb 9, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Nimrit GorayaDonald E Wesson
Aug 17, 2004·Srpski arhiv za celokupno lekarstvo·Biljana Stojimirović, Dejan Petrović

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