Origin, evolution, and mechanism of 5' tRNA editing in chytridiomycete fungi

RNA
M J LaforestB Franz Lang

Abstract

5' tRNA editing has been demonstrated to occur in the mitochondria of the distantly related rhizopod amoeba Acanthamoeba castellanii and the chytridiomycete fungus Spizellomyces punctatus. In these organisms, canonical tRNA structures are restored by removing mismatched nucleotides at the first three 5' positions and replacing them with nucleotides capable of forming Watson-Crick base pairs with their 3' counterparts. This form of editing seems likely to occur in members of Amoebozoa other than A. castellanii, as well as in members of Heterolobosea. Evidence for 5' tRNA editing has not been found to date, however, in any other fungus including the deeply branching chytridiomycete Allomyces macrogynus. We predicted that a similar form of tRNA editing would occur in members of the chytridiomycete order Monoblepharidales based on the analysis of complete mitochondrial tRNA complements. This prediction was confirmed by analysis of tRNA sequences using a tRNA circularization/RT-PCR-based approach. The presence of partially and completely unedited tRNAs in members of the Monoblepharidales suggests the involvement of a 5'-to-3' exonuclease rather than an endonuclease in removing the three 5' nucleotides from a tRNA substrate. Surprisi...Continue Reading

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Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Maria G AbadJane E Jackman
Dec 18, 2012·Nucleic Acids Research·Bhalchandra S RaoJane E Jackman
Oct 13, 2010·Cellular and Molecular Life Sciences : CMLS·Volker Knoop
Mar 4, 2015·International Journal of Molecular Sciences·Thalia Salinas-GiegéPhilippe Giegé
Dec 24, 2014·International Journal of Molecular Sciences·Heike BetatMario Mörl
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Anne-Laure Chateigner-Boutin, Ian Small
Jul 5, 2005·Current Opinion in Microbiology·Charles E Bullerwell, B Franz Lang
Dec 1, 2004·Annual Review of Genetics·Michael W GrayGertraud Burger
Jun 10, 2014·Journal of Theoretical Biology·Massimo Di GiulioBeatrice Cobucci-Ponzano
Aug 4, 2016·Nucleic Acids Research·Yicheng LongJane E Jackman
Oct 30, 2016·RNA Biology·Christiane Rammelt, Walter Rossmanith
Nov 18, 2004·The Journal of Biological Chemistry·Charles E Bullerwell, Michael W Gray
Apr 17, 2014·The Journal of Biological Chemistry·Maria G AbadJane E Jackman
Mar 30, 2012·RNA·Jane E JackmanMichael W Gray

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