PMID: 6160153Dec 25, 1980Paper

Origin of malondialdehyde from DNA degraded by Fe(II) x bleomycin.

The Journal of Biological Chemistry
R M BurgerS B Horwitz

Abstract

Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to ologonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA by Fe(II) x bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

The Journal of Biological Chemistry
R M BurgerS B Horwitz
Proceedings of the National Academy of Sciences of the United States of America
M TakeshitaH Ohtsubo
© 2021 Meta ULC. All rights reserved