Origin of polyproline-rich peptides in human butyrylcholinesterase tetramers

Chemico-biological Interactions
Hong PengO Lockridge

Abstract

The human butyrylcholinesterase (HuBChE) tetramer is composed of 4 identical subunits and a noncovalently bound polyproline-rich peptide. In a previous report we identified lamellipodin as the source of the polyproline-rich peptides in HuBChE tetramers purified from plasma. Our current goal was to identify proteins in addition to lamellipodin that donate polyproline-rich peptides to plasma HuBChE tetramers. Peptides were released from 1 mg of pure plasma-derived HuBChE tetramers by boiling. Mass spectrometry identified 74 polyproline-rich peptides. MALDI-TOF mass spectra and spectral counting of the LC-MS/MS data supported the conclusion that lamellipodin accounted for 70% of the polyproline-rich peptides. Additional precursor proteins were matched through BLASTp searches, suggesting but not proving, that 20 proteins including UDP-N-acetyl glucosamine transferase ALG13 homolog, leiomodin 2, and zinc finger homeobox protein 2 are sources of polyproline-rich peptides found in HuBChE tetramers. Eighteen polyproline-rich peptides had no match in the human protein database. In conclusion, HuBChE assembles into tetramers through interaction of its C-terminal domain with polyproline peptides derived from a variety of proteins.

References

May 15, 1998·The Biochemical Journal·R M BlongO Lockridge
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Citations

Jul 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lawrence M SchopferOksana Lockridge
Oct 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xavier BrazzolottoFlorian Nachon
Feb 23, 2020·Chemico-biological Interactions·Lilly TokerOksana Lockridge
Jun 18, 2018·Archives of Biochemistry and Biophysics·Ashima SaxenaOksana Lockridge

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