Origin of the lag period in the phospholipase C cleavage of phospholipids in membranes. Concomitant vesicle aggregation and enzyme activation

Biochemistry
G BasáñezA Alonso

Abstract

When phospholipase C is added to a suspension of large unilamellar vesicles of egg phosphatidylcholine, maximal rates of hydrolysis occur only after a latency period. No lag period is seen when the substrate is in the form of small (sonicated) vesicles, or of short-chain phosphatidylcholine monomers. For a given vesicle concentration, the lag time may vary as a function of Ca2+, enzyme concentration, or temperature, but activation occurs at a fixed molar fraction of diacylglycerol produced. Lag times decrease gradually with vesicle size, and also with the amount of diacylglycerol present in the bilayers when it is mixed with phospholipid prior to enzyme addition. Parallel recordings of enzyme activity and suspension turbidity reveal that in all cases the latency period ends concomitantly with the start of a process of vesicle aggregation. Both the lag time and the amount of diacylglycerol formed before activation decrease with vesicle concentration, suggesting that enzyme activation is somehow related to vesicle aggregation. The latency period of phospholipase C may be explained in terms of a hypothesis according to which (a) full enzyme activity requires the presence of membrane surface irregularities or defects, (b) the diacy...Continue Reading

Citations

Jul 9, 1999·Progress in Lipid Research·F M Goñi, A Alonso
Oct 23, 1998·Biochimie·J ChopineauM Ollivon
Aug 1, 1997·Current Opinion in Structural Biology·O G Mouritsen, K Jørgensen
Jul 27, 2001·Biochimica Et Biophysica Acta·M Masserini, D Ravasi
May 7, 2009·Annual Review of Biophysics·Banafshé Larijani, Dominic L Poccia
Oct 21, 1999·Journal of Colloid and Interface Science·M B Ruiz-ArgüelloF M Goñi
Apr 17, 2012·Progress in Lipid Research·Félix M GoñiAlicia Alonso
May 12, 2009·Chemistry and Physics of Lipids·Patricia UrbinaFélix M Goni
Dec 31, 2002·Biochemical and Biophysical Research Communications·Qiang He, Junbai Li
Jan 18, 2006·Chemistry and Physics of Lipids·Patricia UrbinaJesús Sot
Dec 23, 2008·Biochimica Et Biophysica Acta·Hasna AhyayauchFélix M Goñi
Nov 16, 2010·Chemistry and Physics of Lipids·David J LópezAlicia Alonso
Mar 15, 2011·Biochimica Et Biophysica Acta·Daniel Otzen
Apr 12, 2008·Biochimica Et Biophysica Acta·Oliver VöglerPablo V Escribá
Jul 19, 2002·Biophysical Journal·Juha M HolopainenPaavo K J Kinnunen
Oct 31, 2006·Biochimica Et Biophysica Acta·Regina AlemanyValentina Ruiz-Gutierrez
Oct 28, 2006·Biochimica Et Biophysica Acta·Pablo V EscribáOliver Vögler
Sep 24, 1999·Transactions of the Royal Society of Tropical Medicine and Hygiene·C BottoJ E Bradley
Jun 4, 2004·The Journal of Biological Chemistry·Ariana BarlicJuan M González-Mañas
Jul 3, 2004·The Journal of Biological Chemistry·Oliver VöglerPablo V Escribá
Aug 23, 2021·Biochimica Et Biophysica Acta. Biomembranes·Marina N IriondoAlicia Alonso
Feb 27, 2001·Biophysical Journal·J M CaaveiroJ M González-Mañas

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