PMID: 702658Oct 1, 1978Paper

Origin of the minor glycoproteins of murine leukemia viruses

Journal of Virology
M C KempR W Compans

Abstract

Polyacrylamide gel electrophoretic analysis and immunoprecipitation were used to study glycoproteins from purified Rauscher murine leukemia virus (R-MuLV) and from AKR thymic lymphoblastoid cell membranes. In addition to gp70, a minor glycoprotein of approximately 52,000 daltons (gp52) was demonstrated in purified R-MuLV preparations, which was antigenically related to gp70. Analysis of R-MuLV glycopeptides obtained after exhaustive Pronase digestion showed that gp70 has at least two different glycopeptide size classes with molecular weights of 5,100 and 2,900, respectively. gp52, however, contained only a single glycopeptide size class of approximately 5,100 daltons, indicating that the two glycoproteins contain distinct carbohydrate components. Trypsin treatment of R-MuLV converted gp70 into a product with a molecular mass of approximately 52,000 daltons as well as a 45,000-dalton minor product, with little effect on virus infectivity. Similarly, trypsin treatment of 125I-labeled glycoproteins derived from AKR mouse lymphoblastoid cell membranes generated fragments antigenically related to gp70 and similar in size to those obtained by trypsin treatment of R-MuLV. In both cases, the appearance of cleavage products was accompan...Continue Reading

References

Jan 1, 1975·The Journal of Experimental Medicine·B C Del VellanoF J Dixon
Oct 1, 1975·Journal of Virology·A Pinter, R W Compans
Dec 1, 1975·Virology·H D KlenkJ Blödorn
Apr 1, 1976·Journal of Virology·S A MoyerD F Summers
Jan 1, 1977·Immunological Reviews·E A Boyse
Apr 1, 1977·Journal of Virology·J A McCarter
Aug 1, 1975·The Journal of Experimental Medicine·R K Zwerner, R T Acton
Sep 1, 1974·Journal of Virology·B M Sefton, K Keegstra
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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