Origin of the retrocorneal membrane in the rabbit

Archives of Ophthalmology
A M Silbert, J L Baum

Abstract

Electron microscopic studies have offered presumptive evidence that the origin of both cellular and extracellular components of the retrocorneal membrane (RCM) derive from corneal endothelial cells. In an attempt to demonstrate conclusively the origin of the cell in the RCM, we performed 8-mm exchange, penetrating keratoplasties between male and female rabbits. After allowing for complete healing and using only clear corneas, we produced central RCM by freezing the central cornea after inducing intraocular inflammation. Care was taken to ensure that the membrane was surrounded by normal donor endothelium and Descemet's membrane was intact. Sex chromatin counts of the cells in the RCM of a female host with a male graft showed an average of 2% Barr bodies. Cells in the membrane of a male host with a female graft showed an average of 40% Barr bodies. The experimental and control sex chromatin counts were almost identical. This study provides conclusive evidence that central retrocorneal, fibroblast-like cells and membranes in rabbits are derived from corneal endothelial cells.

Citations

Jul 15, 1987·American Journal of Ophthalmology·L M HamedE M Helveston
Jan 1, 1983·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·P Enke, J W Rohen
Dec 1, 1986·Current Eye Research·A W TubervilleB J McLaughlin
Sep 1, 1983·Experimental Eye Research·U LabermeierM C Kenney
Jul 1, 1984·Survey of Ophthalmology·D J AppleR J Olson
Jun 17, 2016·Annals of the New York Academy of Sciences·Patrick M McNuttTracey A Hamilton
Jan 1, 1990·Eye·S J Tuft, D J Coster
Apr 30, 1985·Documenta Ophthalmologica. Advances in Ophthalmology·J A WolfeL P Fox
Jun 1, 1992·Acta Ophthalmologica·Z Zagórski, G O Naumann
Jan 13, 2000·Cornea·J Baum
Aug 1, 1983·Seminars in Arthritis and Rheumatism·M E Nimni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.