Origin-Specific Adhesive Interactions of Mesenchymal Stem Cells with Platelets Influence Their Behavior After Infusion

Stem Cells
Lozan SheriffG B Nash

Abstract

We investigated the adhesive behavior of mesenchymal stem cells (MSC) in blood, which might influence their fate when infused as therapy. Isolated human bone marrow MSC (BMMSC) or umbilical cord MSC (UCMSC) adhered efficiently from flow to the matrix proteins, collagen, or fibronectin, but did not adhere to endothelial selectins. However, when suspended in blood, BMMSC no longer adhered to collagen, while UCMSC adhered along with many aggregated platelets. Neither MSC adhered to fibronectin from flowing blood, although the fibronectin surface did become coated with a platelet monolayer. UCMSC induced platelet aggregation in platelet rich plasma, and caused a marked drop in platelet count when mixed with whole human or mouse blood in vitro, or when infused into mice. In contrast, BMMSC did not activate platelets or induce changes in platelet count. Interestingly, isolated UCMSC and BMMSC both adhered to predeposited platelets. The differences in behavior in blood were attributable to expression of podoplanin (an activating ligand for the platelet receptor CLEC-2), which was detected on UCMSC, but not BMMSC. Thus, platelets were activated when bound to UCMSC, but not BMMSC. Platelet aggregation by UCMSC was inhibited by recombina...Continue Reading

References

Apr 23, 2003·Archives of Dermatology·Evangelos V Badiavas, Vincent Falanga
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Jul 29, 2005·Leukemia·J PlumasM-C Favrot
Oct 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Vincent F M SegersGilles W De Keulenaer
Mar 17, 2007·Transplantation Proceedings·S SchrepferM P Pelletier
Jul 10, 2007·The Journal of Biological Chemistry·Katsue Suzuki-InoueYukio Ozaki
Feb 26, 2008·The International Journal of Biochemistry & Cell Biology·Ye ChenGuo-Rong Zhang
Mar 7, 2009·Cell Stem Cell·Jeffrey M Karp, Grace Sock Leng Teo
Mar 31, 2009·Journal of Molecular and Cellular Cardiology·Harald F LangerMeinrad Gawaz
Jun 18, 2009·Inflammation & Allergy Drug Targets·Robert E NewmanAlla Danilkovitch-Miagkova
Jan 1, 2008·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Reinhard HenschlerErhard Seifried
Mar 17, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Victoria AldridgePhilip N Newsome
Apr 24, 2012·Stem Cells·Guido MollKatarina Le Blanc
Jan 15, 2013·Biochemical and Biophysical Research Communications·Kohei TatsumiTeruo Okano
Jun 6, 2013·Acta Pharmacologica Sinica·Xin WeiYu-fang Shi
Jan 16, 2015·Journal of Visualized Experiments : JoVE·Hafsa MunirHelen McGettrick
Mar 20, 2016·British Journal of Anaesthesia·S HeschlM Rigaud
Dec 23, 2017·Nature Communications·Julie RayesSteve P Watson

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Citations

Feb 13, 2019·Journal of Cell Science·Lewis S C WardHelen M McGettrick
Jun 11, 2019·Stem Cells Translational Medicine·Stuart P Atkinson
Oct 3, 2020·International Journal of Molecular Sciences·Vivian Alonso-GarciaFernando A Fierro
Feb 10, 2019·International Journal of Molecular Sciences·Miguel QuintanillaEster Martín-Villar
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Amandine GirousseCoralie Sengenès

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Methods Mentioned

BETA
flow cytometry
Fluorescence

Software Mentioned

Minitab

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