PMID: 16524104Mar 10, 2006Paper

Original paper application of cyclodextrins as modifiers in electrophoretic separation of metalloporphyrins

Journal of Separation Science
Krystyna Pyrzyńska, Krzysztof Kilian

Abstract

Several metallocomplexes of tetrakis-carboxyphenylporphyrin (TCPP) were separated on fused-silica capillary using CZE with UV-VIS detection. Metalloporphyrins of Co(II), Cu(II), Mn(II), Ni(II), and Zn(II) were formed directly in TCPP solution with addition of Cd(II) to increase the formation reaction rate. The composition of BGE, its concentration, and pH were optimized to ensure the stability of complexes and proper resolution. In particular, the problem of signals' shape was investigated and discussed. The presence of beta-CD in borate buffer significantly improved separation efficiency and signal shapes due to formation of inclusion complexes. Under the best separation conditions (50 mM borate running buffer at pH 9 with addition of 2 mM beta-CD, 30 kV applied voltage) a separation of metal complexes with TCPP was accomplished in 16 min.

References

Jun 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·J J SchuitmakerN van Zandwijk
May 26, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S Hamai, T Koshiyama
Jan 19, 2002·Fresenius' Journal of Analytical Chemistry·K Kilian, K Pyrzyñska
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jana CharvátoZdenek Deyl
May 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Krzysztof Kilian, Krystyna Pyrzyńska
Aug 16, 2002·Electrophoresis·Jeremy E Melanson, Charles A Lucy
Dec 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei BuDaniel W Sved
Oct 31, 2008·Talanta·M BiesagaM Trojanowicz

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