Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development

Investigative Ophthalmology & Visual Science
Ying PengJames M Phang

Abstract

In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural retina (gyrate atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, bu...Continue Reading

Citations

Dec 16, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Muthukumaran SivashanmugamSulochana K N
Aug 26, 2018·The Plant Journal : for Cell and Molecular Biology·Changzhen LiuZhukuan Cheng

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