Ornithine aminotransferase activity, tissue ornithine concentrations and polyamine metabolism

The International Journal of Biochemistry
N SeilerB Knödgen

Abstract

1. Inactivation of L-ornithine:2-oxoacid aminotransferase (OAT) by 5-fluoromethylornithine (5FMOrn), a specific inactivator of OAT, causes a great elevation of tissue ornithine (Orn) concentrations. 2. Inhibition of L-ornithine decarboxylase (ODC) by 2-difluoromethylornithine (DFMO) had no effect on Orn concentrations. 3. The combined administration of 5FMOrn and DFMO produced a 2- to 3-fold greater enhancement of tissue Orn concentrations than treatment with 5FMOrn alone. 4. The increase of tissue Orn concentrations had a long-lasting enhancing effect on polyamine metabolism. 5. In the brain this could be demonstrated by the elevation of putrescine and spermidine concentrations and the increase of spermidine turnover rate. 6. In visceral organs polyamine concentrations were not elevated because polyamines can be eliminated by transport. 7. In line with this notion is the fact that urinary polyamine excretion was increased for several days, even after a single dose of 5FMOrn. 8. Inhibitors of 4-aminobutyric acid:2-oxoglutarate aminotransferase which are also inactivators of OAT had the same effect on polyamine excretion as 5FMOrn.

References

Mar 15, 1978·Biochemical and Biophysical Research Communications·D Klein, D R Morris
Feb 1, 1976·The Biochemical Journal·A Herzfeld, S M Raper
Oct 1, 1987·Canadian Journal of Physiology and Pharmacology·N Seiler
Apr 1, 1985·Neurochemical Research·N Seiler, F N Bolkenius
Apr 1, 1985·Journal of Neurochemistry·W W ShinP C Wong
Jan 1, 1985·The Biochemical Journal·N SeilerB Knödgen
May 31, 1985·Journal of Chromatography·N Seiler, B Knodgen
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Russell, S H Snyder
Jan 1, 1981·The International Journal of Biochemistry·N SeilerS Sarhan
Jan 1, 1980·Neurochemical Research·N Seiler, S Sarhan

❮ Previous
Next ❯

Citations

Sep 1, 1993·Metabolic Brain Disease·N Seiler, G Daune-Anglard
Dec 1, 1995·Progress in Neurobiology·R A Kauppinen, L I Alhonen
Mar 1, 1995·The International Journal of Biochemistry & Cell Biology·M Manteuffel-CymborowskaB Grzelakowska-Sztabert
May 27, 1997·Biochimica Et Biophysica Acta·M Manteuffel-CymborowskaB Grzelakowska-Sztabert
Jun 15, 2007·Critical Reviews in Clinical Laboratory Sciences·Nikolaus Seiler, Francis Raul
Feb 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·X HanB A Stanley
Jan 1, 1991·Experimental Parasitology·V SharmaO P Shukla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.