Ornithine-delta-aminotransferase is essential for arginine catabolism but not for proline biosynthesis.

BMC Plant Biology
Dietmar FunckWolfgang Koch

Abstract

Like many other plant species, Arabidopsis uses arginine (Arg) as a storage and transport form of nitrogen, and proline (Pro) as a compatible solute in the defence against abiotic stresses causing water deprivation. Arg catabolism produces ornithine (Orn) inside mitochondria, which was discussed controversially as a precursor for Pro biosynthesis, alternative to glutamate (Glu). We show here that ornithine-delta-aminotransferase (deltaOAT, At5g46180), the enzyme converting Orn to pyrroline-5-carboxylate (P5C), is localised in mitochondria and is essential for Arg catabolism. Wildtype plants could readily catabolise supplied Arg and Orn and were able to use these amino acids as the only nitrogen source. Deletion mutants of deltaOAT, however, accumulated urea cycle intermediates when fed with Arg or Orn and were not able to utilize nitrogen provided as Arg or Orn. Utilisation of urea and stress induced Pro accumulation were not affected in T-DNA insertion mutants with a complete loss of deltaOAT expression. Our findings indicate that deltaOAT feeds P5C exclusively into the catabolic branch of Pro metabolism, which yields Glu as an end product. Conversion of Orn to Glu is an essential route for recovery of nitrogen stored or trans...Continue Reading

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Datasets Mentioned

BETA
W43737

Methods Mentioned

BETA
transgenic
sequence-based prediction
Fluorescence
PCR
genotyping
environmental stress
electrophoresis

Software Mentioned

TargetP
AxioVison
BAR e - northern tool
BAR eFP - Browser

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