Abstract
The natural compound n-butylidenephthalide (BP), which is isolated from the chloroform extract of Angelica sinensis, has been investigated for its antitumoral effects on glioblastoma multiform (GBM) brain tumors both in vitro and in vivo. To determine the mechanism of BP-induced growth arrest and apoptosis, we examined BP-induced changes in gene expression by microarray screening using human GBM brain tumor cells. This analysis identified several BP-inducible genes, including the nuclear receptors NOR-1, Nurr1, and Nur77. Among these genes, Nur77 is particularly interesting because it plays an important role in the apoptotic processes in various tumor cell lines. BP was able to increase Nur77 mRNA and protein expression in a time-dependent manner. After BP treatment in GBM 8401 cells, Nur77 translocated from the nucleus to the cytoplasm, the cytochrome c was released from the mitochondria, and caspase 3 became activated. Furthermore, using Nur77 promoter-luciferase assay, BP increased Nur77 was AP1 related. Inhibition of BP-induced Nur77 expression by Nur77 short interfering RNA blocked BP-induced apoptosis in GBM 8401 cells, suggesting that the induction of Nur77 negatively affected GBM 8401 cell survival. In summary, our resu...Continue Reading
References
Oct 1, 1990·Molecular Endocrinology·A NakaiL J DeGroot
May 1, 1988·Neuron·J Milbrandt
Mar 10, 1995·The Journal of Biological Chemistry·H UemuraC Chang
Dec 1, 1994·Molecular and Cellular Biology·J K Yoon, L F Lau
Jan 20, 1994·Nature·J D WoroniczA Winoto
Jan 20, 1994·Nature·Z G LiuB A Osborne
Oct 1, 1993·Molecular and Cellular Biology·G T Williams, L F Lau
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·F WeihR Bravo
Feb 1, 1997·Seminars in Immunology·A Winoto
Feb 12, 1998·The Journal of Biological Chemistry·Y KatagiriG Guroff
Oct 26, 1999·Science·H D YounJ O Liu
Aug 16, 2000·The EMBO Journal·H D YounJ O Liu
Aug 19, 2000·Science·H LiX Zhang
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alice N McEvoyEvelyn P Murphy
May 2, 2002·Cell·Astar Winoto, Dan R Littman
Jun 5, 2002·World Journal of Gastroenterology : WJG·Su LiuWen-Jin Su
Dec 6, 2003·The EMBO Journal·Arvind RajpalAstar Winoto
Feb 26, 2004·Cell·Bingzhen LinXiao-kun Zhang
Mar 16, 2004·Carcinogenesis·Hye-Jin ShinMi-Ock Lee
May 26, 2004·British Journal of Pharmacology·Yanbin LiangDavid F Woodward
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nu-Man TsaiHorng-Jyh Harn
May 6, 2005·The Journal of Biological Chemistry·Sudhakar ChintharlapalliStephen Safe
May 24, 2005·Cancer Letters·Jussi KoivunenJuha Peltonen
Nov 3, 2005·Experimental & Molecular Medicine·Hyungsoo KimHong Duk Youn
Jan 26, 2006·Oncogene·Y-H HanX-K Zhang
Aug 15, 2006·Biochemical and Biophysical Research Communications·Hyungsoo KimHong-Duk Youn
Sep 22, 2006·Journal of Neurochemistry·Nu-Man TsaiHorng-Jyh Harn
Citations
Jan 27, 2011·Biotechnology Letters·Ru-Huei FuShinn-Zong Lin
Sep 3, 2010·Investigational New Drugs·Po Yen LiuHorng Jyh Harn
Aug 2, 2011·Chinese Journal of Integrative Medicine·Gilberto Ka Kit LeungKwan Ngai Hung
Mar 2, 2011·Molecular Neurobiology·Ramaswamy KannappanBharat Bhushan Aggarwal
Aug 25, 2009·Acta Pharmacologica Sinica·Chyou-wei WeiHorng-jyh Harn
May 14, 2011·Neuro-oncology·Horng-Jyh HarnTzyy-Wen Chiou
Aug 7, 2013·Journal of Biomedical Science·Li-Yi SunChing-Feng Cheng
May 10, 2011·Annals of Surgical Oncology·Po-Cheng LinTzyy-Wen Chiou
Sep 13, 2012·PloS One·Shih-Ping LiuShinn-Zong Lin
Jan 28, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mao-Hsuan HuangHorng-Jyh Harn
Oct 5, 2010·Cell Proliferation·L-Y SunT-W Chiou
Jan 20, 2011·Journal of Surgical Oncology·Li-Fu ChangTzyy-Wen Chiou
Mar 3, 2011·Phytotherapy Research : PTR·Wei-Shin LiuJing-Ren Jeng
Dec 3, 2014·Molecular and Cellular Endocrinology·Ling LiVincent Laudet
Apr 28, 2012·Expert Opinion on Therapeutic Targets·Sally K Y ToAlice S T Wong
Jun 19, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Cheng-Yoong PangHsueh-Hui Yang
Jan 4, 2015·Steroids·Alicja PawlakWojciech Kalas
Aug 11, 2015·Oncogene·S-Y YenT-W Chiou
Feb 4, 2016·PloS One·Alexa TengaTaosheng Chen
Jan 24, 2017·IUBMB Life·Sayeed Ur RehmanMohammad Tabish
Feb 18, 2017·International Journal of Molecular Sciences·Ssu-Yin YenHorng-Jyh Harn
Oct 5, 2010·Cell Transplantation·Po-Cheng LinHorng-Jyh Harn
Feb 2, 2018·International Journal of Molecular Sciences·Hueng-Chuen FanHorng-Jyh Harn
Nov 6, 2009·Acta Pharmacologica Sinica·Dong XuGuang-mei Yan
Dec 24, 2019·Bioscience Trends·Jinjing WangLin Zhao
Apr 17, 2012·Molecular BioSystems·Jie YangYing-Jin Yuan
Jan 22, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Ching-Yi Wu, Yu-Chie Chen
Sep 30, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·X-F HuangN-M Tsai
Jun 25, 2020·International Journal of Molecular Sciences·En-Yi LinTzyy-Wen Chiou
Dec 7, 2017·BMC Complementary and Alternative Medicine·Sheng-Chun ChiuTeng-Fu Hsieh
Oct 23, 2020·Cancers·Peter Kok-Ting WanHextan Yuen-Sheung Ngan
Nov 11, 2018·The American Journal of Pathology·Asoka BannoRaju C Reddy
May 3, 2021·Bioorganic Chemistry·Xiaoyu DingHeng Xu
Jan 2, 2010·Journal of Agricultural and Food Chemistry·Yung-Luen YuYi-Lin Sophia Chen