Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Nature Methods
Kevin M EsveltGeorge M Church

Abstract

The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas acquired immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but it can mediate only a single activity at a time within any given cell. Here we characterize a set of fully orthogonal Cas9 proteins and demonstrate their ability to mediate simultaneous and independently targeted gene regulation and editing in bacteria and in human cells. We find that Cas9 orthologs display consistent patterns in their recognition of target sequences, and we identify an unexpectedly versatile Cas9 protein from Neisseria meningitidis. We provide a basal set of orthogonal RNA-guided proteins for controlling biological systems and establish a general methodology for characterizing additional proteins.

References

Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y G KimSrinivasan Chandrasegaran
Jun 28, 2005·Nucleic Acids Research·Andreas GroteDieter Jahn
Aug 6, 2005·Nature Biotechnology·Matthew Porteus, Dana Carroll
Dec 11, 2007·Journal of Bacteriology·Philippe HorvathRodolphe Barrangou
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 23, 2009·Nature Reviews. Molecular Cell Biology·Priscilla E M Purnick, Ron Weiss
Nov 26, 2009·Science·Matthew J Moscou, Adam J Bogdanove
Apr 2, 2011·Nature·Elitza DeltchevaEmmanuelle Charpentier
Nov 9, 2011·Annual Review of Genetics·Devaki BhayaRodolphe Barrangou
Feb 18, 2012·Nature·Blake WiedenheftJennifer A Doudna
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Dec 18, 2012·Nature·Joe Bondy-DenomyAlan R Davidson
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Jan 29, 2013·Current Opinion in Microbiology·Anna I Podgornaia, Michael T Laub
Jan 31, 2013·Nature Biotechnology·Wenyan JiangLuciano A Marraffini
Jan 31, 2013·Nature Biotechnology·Seung Woo ChoJin-Soo Kim
May 15, 2013·Trends in Biotechnology·Thomas GajCarlos F Barbas
Jun 14, 2013·Nucleic Acids Research·David BikardLuciano A Marraffini
Jul 31, 2013·Nature Methods·Pablo Perez-PineraCharles A Gersbach
Jul 31, 2013·Nature Methods·Morgan L MaederJ Keith Joung

Citations

May 9, 2014·Genetics·Juliane ZantkeKristin Tessmar-Raible
Mar 1, 2014·Nature Methods·Ryan Forster, Dirk Hockemeyer
May 28, 2014·Glycobiology·Catharina SteentoftHenrik Clausen
May 2, 2014·Nature Methods·Jennifer A N Brophy, Christopher A Voigt
Apr 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peter C FineranStan J J Brouns
Apr 29, 2014·Nature Biotechnology·Shengdar Q TsaiJ Keith Joung
May 6, 2014·Annales d'endocrinologie·Denise R Archambeault, Martin M Matzuk
Jul 25, 2014·Nature Protocols·Hui YangRudolf Jaenisch
Sep 30, 2014·Nature Methods·Charles A Gersbach
Oct 2, 2014·The Journal of Clinical Investigation·Rajat M Gupta, Kiran Musunuru
Sep 5, 2014·Microbial Cell Factories·Jan Peter van Pijkeren, Robert A Britton
Jul 19, 2014·ELife·Kevin M EsveltGeorge M Church
Jul 6, 2014·Bioinformatics·Marc GuellGeorge M Church
Mar 4, 2014·Nature Biotechnology·Jeffry D Sander, J Keith Joung
Aug 15, 2014·Nucleic Acids Research·Ami M KabadiCharles A Gersbach
Feb 28, 2015·Quantitative Biology·Xuebing WuPhillip A Sharp
Oct 30, 2014·Nature Communications·Jake Z JacobsMikel Zaratiegui
Jan 23, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael T Certo, Richard A Morgan
Jan 12, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ciaran M LeeGang Bao
Jan 20, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ciaran M LeeGang Bao
Feb 9, 2016·Nature Chemical Biology·Matthew F CopelandBrian F Pfleger
Jan 15, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Versha PrakashRafael J Yáñez-Muñoz
Dec 29, 2015·Metabolic Engineering·Tadas JakočiūnasJay D Keasling
Dec 3, 2015·Current Opinion in Biotechnology·Rodolphe Barrangou, Jan-Peter van Pijkeren
Oct 31, 2015·Biotechnology Advances·Gert PetersMarjan De Mey
Dec 29, 2015·Current Opinion in Biotechnology·Oleg Raitskin, Nicola J Patron
Jan 29, 2016·Nature Methods·Pratiksha I ThakoreCharles A Gersbach
Jan 30, 2016·Plant Methods·Simon Schiml, Holger Puchta
Dec 24, 2014·Biotechnology Advances·Luisa Bortesi, Rainer Fischer
Jan 27, 2016·Trends in Biotechnology·Barbara JusiakTimothy K Lu
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wulan DengRobert H Singer
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hanhui MaThoru Pederson
Jan 22, 2015·Immunity·Stephane PelletierDouglas R Green
Nov 19, 2015·The Plant Journal : for Cell and Molecular Biology·Jeannette SteinertHolger Puchta
Dec 15, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maximilian MüllerClaudio Mussolino
Feb 18, 2016·Stem Cells International·Bing ShuiYing Peng
Nov 14, 2015·Annual Review of Pharmacology and Toxicology·Matthew Porteus
Nov 27, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Prajit LimsirichaiDavid V Schaffer
Dec 31, 2015·Nature Methods·Mehmet Fatih BolukbasiScot A Wolfe
Mar 6, 2016·Trends in Biotechnology·Ioannis MougiakosJohn van der Oost
Dec 25, 2015·Genome Biology·Jennifer A Doudna, Charles A Gersbach
Dec 17, 2015·Nature Reviews. Molecular Cell Biology·Antonia A DominguezLei S Qi
Feb 28, 2015·Current Opinion in Structural Biology·Fuguo Jiang, Jennifer A Doudna
Jun 16, 2015·ACS Synthetic Biology·Gabriel Lopez, J Christopher Anderson
Oct 11, 2015·Current Opinion in Biotechnology·Brady F CressMattheos A G Koffas
Nov 8, 2015·Current Opinion in Cell Biology·Joshua Z Johnson, Dirk Hockemeyer
Oct 31, 2015·Current Opinion in Chemical Biology·Henriette O'GeenDavid J Segal
Dec 10, 2015·Nature Biotechnology·Erik J Sontheimer, Scot A Wolfe
Nov 3, 2015·Nature Biotechnology·Benjamin P KleinstiverJ Keith Joung
Sep 8, 2015·Current Opinion in Pharmacology·Claudio MussolinoToni Cathomen
Mar 19, 2014·Current Opinion in Biotechnology·Matthew F CopelandBrian F Pfleger
Nov 7, 2014·Molecular Cell·Alexandra E BrinerRodolphe Barrangou
Sep 17, 2015·Nature·Matthew C CanverDaniel E Bauer
Oct 6, 2015·Nature Biotechnology·James E DahlmanSilvana Konermann
Nov 7, 2015·Molecular Cell·Enbo MaJennifer A Doudna
Sep 8, 2015·Nature Methods·Samira KianiGeorge M Church
Oct 16, 2015·Biotechnology and Bioengineering·Michelle L LuoChase L Beisel
May 12, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Dandan ZhangJian-Feng Li
Oct 17, 2015·Molecular Cell·Yan ZhangErik J Sontheimer
Mar 22, 2016·Cell·David A NellesGene W Yeo
Mar 24, 2016·Cancer Letters·Volkan I Sayin, Thales Papagiannakopoulos
Jan 7, 2014·Development, Growth & Differentiation·Tetsushi Sakuma, Knut Woltjen
Dec 11, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Timothy R Sampson, David S Weiss
Mar 8, 2014·The Plant Journal : for Cell and Molecular Biology·Orlando de LangeThomas Lahaye
Mar 22, 2016·ACS Synthetic Biology·Manish KushwahaAlfonso Jaramillo
Oct 9, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Koen J T VenkenKristi L Hoffman
May 21, 2015·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Michael V WilesHaoyi Wang
Nov 21, 2015·Genome Biology·Tautvydas KarvelisVirginijus Siksnys
Nov 19, 2015·Genome Biology·Robert D FagerlundPeter C Fineran
Apr 5, 2016·Molecular Cell·Ryan T LeenayChase L Beisel
May 6, 2014·Biochimica Et Biophysica Acta·Tobias WijshakeBart van de Sluis
Aug 25, 2015·Microbiology·Rosemary Sanozky-DawesRodolphe Barrangou
Feb 22, 2014·Trends in Genetics : TIG·Rebecca M Terns, Michael P Terns
Sep 22, 2015·Epigenetics & Chromatin·Benjamin I Laufer, Shiva M Singh
Jul 21, 2015·Metabolic Engineering·Monica P McNerneyMark P Styczynski
Apr 17, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David A NellesGene W Yeo
Mar 3, 2015·The FEBS Journal·Ella Hartenian, John G Doench
May 20, 2015·Hearing Research·Bing ZouXuezhong Liu
Jul 23, 2014·Current Opinion in Microbiology·Emmanuelle Charpentier, Luciano A Marraffini
Feb 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Andrew R Bassett, Ji-Long Liu
Jun 18, 2015·Nucleic Acids Research·Dong-Jiunn Jeffery TruongOskar Ortiz
Jun 23, 2015·Nature·Benjamin P KleinstiverJ Keith Joung
Jun 13, 2015·Human Gene Therapy·Jennifer L GoriDavid Bumcrot
Apr 17, 2016·Plant Cell Reports·Jeannette SteinertHolger Puchta
Jun 17, 2015·Human Gene Therapy·Erik J Sontheimer, Rodolphe Barrangou
Jun 5, 2015·Nature Reviews. Cancer·Francisco J Sánchez-Rivera, Tyler Jacks
Jun 7, 2015·Annual Review of Biophysics·Tsz Kin Martin Tsui, Hong Li
Mar 6, 2015·Clinical Epigenetics·Tomasz P JurkowskiPeter Stepper
Jun 9, 2015·Frontiers in Immunology·Lisa M Ott de BruinKiran Musunuru
Jun 11, 2015·Genome Medicine·Haiwei MouWen Xue
Feb 26, 2015·Journal of Medical Genetics·Lu Xiao-JieJi Li-Juan
May 23, 2015·Molecular Cell·Samuel H Sternberg, Jennifer A Doudna
May 23, 2015·Molecular Cell·Michael Boettcher, Michael T McManus
Feb 24, 2015·Trends in Microbiology·Kurt Selle, Rodolphe Barrangou
Apr 20, 2016·Critical Reviews in Biotechnology·Agnieszka A Piatek, Magdy M Mahfouz
Apr 15, 2015·Omics : a Journal of Integrative Biology·Subin Raj Cheri Kunnumal RajendranAnil Kumar
Mar 17, 2015·Nature Methods·Nicola A KearnsRené Maehr
Jul 11, 2014·Current Opinion in Biotechnology·Joseph K Cheng, Hal S Alper
Mar 31, 2015·Trends in Biotechnology·Ignazio Maggio, Manuel A F V Gonçalves
Mar 29, 2014·Trends in Plant Science·Jorge Lozano-Juste, Sean R Cutler
Apr 10, 2015·Nature Reviews. Genetics·Ophir ShalemFeng Zhang
Jun 8, 2014·Current Opinion in Plant Biology·Adam J Bogdanove
Mar 19, 2014·Current Opinion in Microbiology·Peter C Fineran, Ron L Dy
Jan 3, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Qurrat Ul AinYong-Hee Kim
Mar 3, 2015·Nature Methods·Alejandro ChavezGeorge M Church
Apr 29, 2014·Frontiers in Cellular and Infection Microbiology·Timothy R Sampson, David S Weiss
Jan 8, 2015·Frontiers in Bioengineering and Biotechnology·Alessandro LaganàCarlo Maria Croce
Apr 27, 2016·Plant Cell Reports·Joseph W Paul, Yiping Qi
Oct 20, 2014·Nature Biotechnology·Lukasz SwiechFeng Zhang
Jul 21, 2014·Cellular and Molecular Life Sciences : CMLS·Kun XuZhiying Zhang
Sep 23, 2014·Trends in Cell Biology·Andrew C White, William E Lowry
May 12, 2016·Nucleic Acids Research·Juan C OliverosFlorencio Pazos
May 18, 2016·Progress in Retinal and Eye Research·Sandy S C HungAlex W Hewitt
May 26, 2016·Seminars in Cell & Developmental Biology·Krysta L EngelDaniel Savic
May 29, 2016·International Journal of Hematology·Mark J OsbornDaniel F Voytas
Jun 9, 2016·Human Genetics·Daniel StoneKeith R Jerome
May 6, 2016·Annual Review of Biochemistry·Haifeng WangLei S Qi
Jun 28, 2016·Current Opinion in Biotechnology·Andriy DidovykJeff Hasty
Jul 7, 2016·Nature Reviews. Molecular Cell Biology·Rui LopesReuven Agami
May 6, 2016·Annual Review of Biophysics·Baohui ChenBo Huang
Jul 28, 2016·Current Opinion in Microbiology·Robert W BradleyBaojun Wang
Aug 29, 2016·Advanced Drug Delivery Reviews·I Cody MacDonald, Tara L Deans
Sep 7, 2016·Nature Methods·Wei Leong ChewGeorge M Church
Sep 9, 2016·Nature Biotechnology·Rodolphe Barrangou, Jennifer A Doudna
Sep 10, 2016·Nature Reviews. Cardiology·Alanna Strong, Kiran Musunuru
Oct 14, 2016·Nucleic Acids Research·Chance M NowakLeonidas Bleris
Nov 12, 2016·Gene·Vijai SinghPawan Kumar Dhar
Nov 30, 2016·Molecular Therapy. Nucleic Acids·Xianglian GeFeng Gu
Dec 2, 2016·Regenerative Medicine·Kuang-Yui Chen, Paul S Knoepfler
Nov 20, 2016·Cellular Microbiology·Ferdy R van Diemen, Robert Jan Lebbink
Jan 28, 2017·Journal of Huntington's Disease·Tuyana B MalankhanovaSuren M Zakian
Jan 30, 2016·PLoS Computational Biology·Iman Farasat, Howard M Salis
Dec 23, 2016·Nature·David BursteinJillian F Banfield
Dec 2, 2016·Nucleic Acids Research·Peter StepperTomasz P Jurkowski
Feb 22, 2017·Nature Communications·Eunji KimJin-Soo Kim
Feb 6, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Steven HavlicekLawrence W Stanton
Mar 10, 2017·ACS Synthetic Biology·Tsz Kin Martin TsuiHong Li
Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jayme Salsman, Graham Dellaire
Mar 23, 2017·ACS Synthetic Biology·Marie-Laurence LemaySylvain Moineau
Apr 9, 2017·Applied Microbiology and Biotechnology·Ashraf S A El-SayedGul Shad Ali
Apr 4, 2017·Pathogens and Disease·Yan Zhang
May 23, 2017·Nature Communications·Gianluca PetrisAnna Cereseto
Apr 5, 2017·Annual Review of Biophysics·Fuguo Jiang, Jennifer A Doudna
May 26, 2017·Journal of Cellular Biochemistry·Xing-Da JuZhong Sheng Sun
Mar 4, 2017·Nature Communications·Quentin R V FerryTudor A Fulga
Jun 29, 2017·ACS Synthetic Biology·Pan TaoVenigalla Rao
Feb 1, 2017·RNA Biology·Mario Andrea Marchisio, Zhiwei Huang
Jun 18, 2017·The Journal of Gene Medicine·Puping LiangJunjiu Huang
Oct 27, 2017·International Journal of Molecular Sciences·Hong Jo LeeJae Yong Han
Nov 1, 2017·International Journal of Molecular Sciences·Wataru FujiiKunihiko Naito
Dec 20, 2017·Genes·Tapan Kumar MohantaHanhong Bae
Nov 13, 2014·Nucleic Acids Research·Summer B ThymeDavid Baker
Oct 19, 2014·Nucleic Acids Research·Michelle L LuoChase L Beisel
Dec 22, 2017·Nucleic Acids Research·Maurice LabuhnDirk Heckl
Aug 31, 2017·Fly·Alexandra FranzKonrad Basler
Feb 2, 2018·FEBS Letters·Evan A HeiderscheitAseem Z Ansari
Oct 29, 2017·Angewandte Chemie·Spencer C KnightJennifer A Doudna
Nov 2, 2017·Expert Review of Anti-infective Therapy·Marta TrevisanLuisa Barzon
Feb 14, 2018·Biotechnology Journal·Jiazhang LianHuimin Zhao
Mar 27, 2018·The Plant Journal : for Cell and Molecular Biology·Felix Wolter, Holger Puchta
Apr 6, 2018·Chemical Science·Irene Lostalé-SeijoJavier Montenegro
Nov 24, 2017·Frontiers in Plant Science·Leena Arora, Alka Narula
Nov 15, 2017·Nature Communications·Emily A SaundersonGabriella Ficz
Nov 22, 2017·Nature Communications·Nan HaoIan B Dodd
Nov 24, 2017·Nature Communications·Jiazhang LianHuimin Zhao
Dec 6, 2017·Scientific Reports·Jeffrey M Spencer, Xiaoliu Zhang
Jul 22, 2017·Journal of Cellular Biochemistry·Rea Globus, Udi Qimron
Jan 31, 2014·Nature·Samuel H SternbergJennifer A Doudna
Apr 8, 2016·Blood·Matthew C Canver, Stuart H Orkin
Jun 20, 2018·Biotechnology Journal·Katia TarasavaRyan T Gill
Aug 6, 2018·Current Microbiology·Muhammad R JavedAnam Ijaz
Sep 30, 2017·Pediatric Pulmonology·Erica A Roesch, Mitchell L Drumm
Aug 11, 2017·Critical Reviews in Biotechnology·Avik BanerjeePratyoosh Shukla
Aug 5, 2018·Biotechnology Journal·Brian J Mendoza, Cong T Trinh
Aug 22, 2018·Expert Review of Respiratory Medicine·Oliver J McElvaneyNoel G McElvaney
Apr 26, 2015·Applied and Environmental Microbiology·Tao XuJizhong Zhou
Jan 7, 2014·Applied and Environmental Microbiology·Tao XuJizhong Zhou
Aug 26, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Alexandra C Chadwick, Kiran Musunuru
Sep 1, 2016·Journal of the National Cancer Institute·Christina GeblerFrank Buchholz
Mar 20, 2018·F1000Research·Joke TerrynCatherine Verfaillie
Nov 3, 2018·Journal of Integrative Plant Biology·Jochen KumlehnHolger Puchta
Jan 24, 2019·Cellular and Molecular Life Sciences : CMLS·Dmitry KostyushevVladimir Chulanov
Jan 20, 2018·G3 : Genes - Genomes - Genetics·Emily RoggenkampGregory C Finnigan
Mar 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hyojin KimMartin Fussenegger
Dec 19, 2015·Genetics·Ryan T BellAndrew Z Fire
Apr 16, 2019·Nucleic Acids Research·Mareike D HoffmannDominik Niopek
Apr 9, 2013·Nature Methods·Natalie de Souza
Apr 24, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sudhanshu P RaikwarAsgar Zaheer
Apr 17, 2014·Nature Reviews. Microbiology·Lei S Qi, Adam P Arkin
Jan 31, 2019·Annual Review of Immunology·Dimitre R Simeonov, Alexander Marson
Oct 1, 2013·Nature Methods·Prashant MaliGeorge M Church
Dec 5, 2017·Nature Reviews. Genetics·John G Doench
Jun 19, 2019·Chemical Communications : Chem Comm·Emily A Berckman, Wilfred Chen
May 30, 2019·The Journal of Biological Chemistry·Da-Wei LinPeter Kaiser
Jul 19, 2019·Journal of Cellular Physiology·Niaz AhmadBaohong Zhang
May 31, 2019·Nature Reviews. Molecular Cell Biology·Adrian Pickar-Oliver, Charles A Gersbach
Nov 26, 2014·Molecular Systems Biology·Alec A K Nielsen, Christopher A Voigt
Jul 17, 2019·Nature Plants·Yingxiao ZhangYiping Qi
Nov 29, 2014·Science·Jennifer A Doudna, Emmanuelle Charpentier
Oct 30, 2015·Virologica Sinica·Cheng PengDongliang Yang
Apr 2, 2016·Science·Alec A K NielsenChristopher A Voigt
Jun 22, 2017·Annual Review of Biomedical Engineering·Joshua B BlackCharles A Gersbach
Jan 5, 2020·Genome Research·Daniel AgudeloYannick Doyon
Nov 7, 2018·Science Advances·Pranam ChatterjeeJoseph M Jacobson
Aug 20, 2016·Science·Nili OstrovGeorge M Church
Jan 26, 2020·International Journal of Molecular Sciences·Quynh NguyenToshifumi Yokota
Feb 11, 2020·Journal of Cellular and Molecular Medicine·Jingyi WangBo Feng
Apr 25, 2020·Nucleic Acids Research·Thomas JacobsenChase L Beisel
May 1, 2019·Nature Communications·Seiichi HiranoOsamu Nureki
Aug 17, 2016·Oncotarget·Benjamin Garcia-BlojPilar Blancafort
Aug 19, 2020·PLoS Neglected Tropical Diseases·Wei HuangMarcelo Jacobs-Lorena
Sep 24, 2020·Biochemical Society Transactions·Javier Santos-Moreno, Yolanda Schaerli
Jan 1, 2015·Current Protocols in Stem Cell Biology·Susan M Byrne, George M Church
Feb 22, 2017·Current Cardiology Reports·Alexandra C Chadwick, Kiran Musunuru
May 28, 2017·Current Atherosclerosis Reports·Alexandra C Chadwick, Kiran Musunuru
Jul 29, 2017·Scientific Reports·Tina XiongCarl D Novina
Jun 2, 2018·Neural Plasticity·Carla Sanjurjo-Soriano, Vasiliki Kalatzis
Sep 1, 2018·Nucleic Acids Research·Brendan J Hussey, David R McMillen
Aug 3, 2018·Scientific Reports·Alexandra B CrawleyRodolphe Barrangou
Mar 19, 2019·Annual Review of Biochemistry·Daesik KimJin-Soo Kim
Apr 4, 2019·Molecular Biology Reports·Rakesh KumarG P Singh
Apr 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Charleston NobleKevin M Esvelt
Apr 21, 2019·FEMS Microbiology Letters·Thomas JacobsenChase L Beisel
Sep 11, 2019·Scientific Reports·Wataru FujiiKunihiko Naito
May 10, 2018·Biology Methods and Protocols·Tobias AntonSebastian Bultmann
Feb 12, 2020·Nature Biotechnology·Shannon M MillerDavid R Liu
Jun 24, 2020·Nature Biotechnology·Andrew V AnzaloneDavid R Liu
Jul 23, 2020·Frontiers in Bioengineering and Biotechnology·Wentao DingShuobo Shi
Sep 26, 2020·Frontiers in Plant Science·Solmaz KhosraviAndreas Houben
Mar 19, 2014·The Journal of Biological Chemistry·Hongfan ChenScott Bailey
Jun 2, 2016·The FEBS Journal·Suhani VoraGeorge M Church
Jul 3, 2016·Cold Spring Harbor Protocols·Alexandra E BrinerRodolphe Barrangou
Nov 30, 2016·Frontiers in Plant Science·Levi LowderYiping Qi
Jun 6, 2017·Nature Microbiology·Rodolphe Barrangou, Philippe Horvath
Oct 13, 2016·Annual Review of Genetics·Alan S L WongTimothy K Lu
Feb 1, 2017·Bioengineered·Shota NakadeTetsushi Sakuma
Jun 6, 2017·Nature Biotechnology·Linyi GaoFeng Zhang
Apr 30, 2017·Cell & Bioscience·Aimee MalzahnYiping Qi
Aug 1, 2017·Nature Plants·Kangquan YinJin-Long Qiu
Feb 13, 2018·Stem Cells International·Benedetta M MottaAlessandra Rossini
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alejandro ChavezGeorge M Church
Nov 12, 2017·Nature Communications·Lucas B HarringtonJennifer A Doudna
Dec 1, 2017·Current Microbiology·Hadi BayatAzam Rahimpour
Jun 3, 2018·Nature Reviews. Molecular Cell Biology·Mingqi Xie, Martin Fussenegger
Dec 7, 2018·Genome Biology·Nadia AmraniErik J Sontheimer
Mar 20, 2019·Frontiers in Cell and Developmental Biology·Kaili LiuGaurav K Varshney
Nov 20, 2018·Nature Communications·Mehmet Fatih BolukbasiScot A Wolfe
Apr 26, 2019·The CRISPR Journal·Louise RobertsonPaul Q Thomas
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sundaram AcharyaDebojyoti Chakraborty
Jun 15, 2019·Life Science Alliance·Erich BrunnerKonrad Basler
Dec 4, 2019·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Solmaz KhosraviAndreas Houben
Mar 29, 2020·Science·Russell T WaltonBenjamin P Kleinstiver
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Ksenia M ShakirovaErdem B Dashinimaev
Aug 22, 2020·PeerJ·Zachary J WaldripAlan J Tackett
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Asuka EguchiAseem Z Ansari
May 6, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Toshitsugu FujitaHodaka Fujii
Apr 19, 2016·Nature Biotechnology·Hanhui MaThoru Pederson
Feb 28, 2017·BMC Medicine·Jessica L SchnellerCharles P Venditti
Jan 6, 2018·Genetics·Ethan BierJill Wildonger
Oct 5, 2016·Cold Spring Harbor Protocols·Charles E VejnarAntonio J Giraldez
Aug 30, 2018·International Journal of Genomics·Lucas F RibeiroRichard J Ward
Dec 15, 2018·Natural Product Reports·Yaojun TongSang Yup Lee
May 29, 2018·Drug Delivery·Christopher A LinoJerilyn A Timlin
Nov 14, 2018·Bioinformatics·Jiecong Lin, Ka-Chun Wong
Nov 30, 2018·Journal of Hematology & Oncology·Juanjuan ZhaoDelong Liu
May 11, 2019·Nature Communications·Kale KundertTanja Kortemme
Dec 21, 2019·Nature Communications·Jiazhang LianHuimin Zhao
Nov 10, 2015·Genome Biology·Rodolphe Barrangou
Dec 10, 2016·Cold Spring Harbor Protocols·Hannah K RatnerDavid S Weiss
May 28, 2016·Scientific Reports·Siyuan WangXiaowei Zhuang
May 2, 2018·Therapeutic Innovation & Regulatory Science·Jorge E SimónNelson Santiago Vispo
May 31, 2019·International Journal of Molecular Sciences·Iris KoeppelJochen Kumlehn
Jun 26, 2018·Advanced Science·Yinnan Chen, Yanmin Zhang
Jun 26, 2020·MSphere·Cas Mosterd, Sylvain Moineau
Apr 30, 2020·International Journal of Molecular Sciences·Xingbo XuElisabeth M Zeisberg
Jul 3, 2016·Cold Spring Harbor Protocols·Alexandra E Briner, Rodolphe Barrangou
Jun 22, 2017·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Alberto Cebrian-Serrano, Benjamin Davies
Dec 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jason M WolfsDavid R Edgell
Dec 2, 2016·Cold Spring Harbor Perspectives in Biology·Thomas GajJia Liu
Oct 7, 2020·Nature Reviews. Drug Discovery·Navneet Matharu, Nadav Ahituv
Nov 6, 2020·Nucleic Acids Research·Iana FedorovaKonstantin Severinov
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Ning GaoHuihui Liu
Oct 16, 2020·Gene·Ekaterina KondratevaSvetlana Smirnikhina
Jan 10, 2021·Science China. Life Sciences·Zhiquan LiuZhanjun Li
Jul 17, 2020·Computational and Structural Biotechnology Journal·Gue-Ho HwangSangsu Bae
Jul 1, 2020·Advanced Drug Delivery Reviews·Wenhan Yu, Zhijian Wu
Jan 24, 2021·Nature Communications·Daphne Collias, Chase L Beisel
Oct 28, 2020·Scientific Reports·Michelle M DuongSam R Nugen
May 31, 2019·Emerging Topics in Life Sciences·Suk See De Ravin, Julie Brault
Nov 4, 2020·Nature Communications·Giedrius GasiunasVirginijus Siksnys
Aug 29, 2017·Cell·Lucas B HarringtonJennifer A Doudna
Jun 17, 2018·Plant Science : an International Journal of Experimental Plant Biology·Agnieszka A PiatekC Neal Stewart
Dec 6, 2016·Journal of Molecular Biology·Ryan T Leenay, Chase L Beisel
Sep 24, 2018·Cell Systems·John SelbergMarco Rolandi
Dec 17, 2016·Analytical Biochemistry·F Ann Ran
Apr 8, 2021·Nature·Krishanu SahaSCGE Consortium
Apr 12, 2021·Methods : a Companion to Methods in Enzymology·Anastasiya KostyushevaVladimir Chulanov
Apr 10, 2021·Journal of Zhejiang University. Science. B·Chao LiBaohong Zhang
Oct 23, 2014·Development·Ying PengStephen C Ekker
Dec 12, 2017·Cell·Hsin-Kai LiaoJuan Carlos Izpisua Belmonte
Nov 7, 2020·Cell Stem Cell·Delilah HendriksBenedetta Artegiani

Methods Mentioned

BETA
PCR
transfection
transfections

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