Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity.

Immunity
Tyler J RippergerDeepta Bhattacharya

Abstract

We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection.

Associated Datasets

Oct 4, 2020·Deepta Bhattacharya

Citations

Dec 15, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philippe SelhorstKevin K Ariën
Dec 29, 2020·Science Immunology·Catherine J ReynoldsRosemary J Boyton
Jan 5, 2021·Frontiers in Immunology·Yannick GalipeauMarc-André Langlois
Jan 7, 2021·Immunity·Nicholas N JarjourStephen C Jameson
Jan 16, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Antonin BalUNKNOWN COVID SER Study Group
Feb 5, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jintao ZhangLiang Dong
Nov 29, 2020·BMJ : British Medical Journal·Roberto Pastor-BarriusoUNKNOWN ENE-COVID Study Group
Jan 19, 2021·Nature·Christian GaeblerMichel C Nussenzweig
Jan 27, 2021·Cell·Alessandro Sette, Shane Crotty
Jan 5, 2021·Frontiers in Medicine·Juliana GonçalvesHelena Soares
Jan 1, 2021·PloS One·Nathan PostSharif A Ismail
Jan 10, 2021·Cellular & Molecular Immunology·Pai PengAi-Long Huang
Jan 27, 2021·Emerging Infectious Diseases·Sriram SelvarajuUNKNOWN Chennai COVID Sero-Surveillance Team
Jan 23, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Arnaud G L'HuillierUNKNOWN Geneva Centre for Emerging Viral Diseases
Jan 7, 2021·Nature Reviews. Immunology·Marc Veldhoen, J Pedro Simas
Jan 31, 2021·The Lancet Global Health·Manoj V MurhekarUNKNOWN ICMR Serosurveillance Group
Feb 12, 2021·Cell·Aurélien SokalMatthieu Mahévas
Feb 9, 2021·The World Allergy Organization Journal·Alessandro Fiocchi, Erika Jensen-Jarolim
Feb 24, 2021·Journal of the American Heart Association·Abdulla A DamlujiChristopher deFilippi
Feb 28, 2021·Journal of the American Society of Nephrology : JASN·Hamza SakhiUNKNOWN Mondor NephroCov Study Group
Jan 4, 2021·Diagnostic Microbiology and Infectious Disease·Erika P OrnerChristopher W Farnsworth
Feb 27, 2021·The British Journal of General Practice : the Journal of the Royal College of General Practitioners·Azmaeen ZarifSimon de Lusignan
Feb 22, 2021·Advanced Drug Delivery Reviews·Donald Forthal
Mar 12, 2021·The Lancet Global Health·Manoj V Murhekar, Hannah Clapham
Mar 19, 2021·American Journal of Public Health·William RileyJeffrey McCullough
Mar 17, 2021·Nature Reviews. Immunology·Thiago CarvalhoAkiko Iwasaki
Mar 15, 2021·Cytokine & Growth Factor Reviews·Maurizio Federico
Mar 23, 2021·Cell Reports Medicine·Arunasingam AbayasingamUNKNOWN COSIN Study Group
Mar 23, 2021·Clinical & Translational Immunology·Alana L WhitcombeNicole J Moreland
Mar 27, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Michael C WehrhahnJennifer Robson
Mar 26, 2021·Health Systems·Paul R HarperThomas E Woolley
Apr 11, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Mario PoljakTatjana Avšič Županc
Apr 27, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Amanda RadulescuMihaela Lupse
Apr 27, 2021·American Journal of Clinical Pathology·Daniel GeislerSarah E Wheeler

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISAs
ELISA

Key Resources (RRID) Mentioned

Addgene_
Addgene

Software Mentioned

GraphPad Prism
Prism
Microsoft excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.