PMID: 8950593Nov 1, 1996Paper

Orthotopic corneal transplantation in the mouse--a new surgical technique with minimal endothelial cell loss

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
E P ZhangF Hoffmann

Abstract

The murine model of orthotopic perforating keratoplasty is important for studying the allograft reaction, but the small dimensions cause technical difficulties. The anterior chamber of the eye of the BALB/c mouse was measured with the confocal microscope and with histological methods. Ten C3H mouse donor corneas each were separated by the conventional technique and by the newly developed "underwater" technique, where the opened donor eye did not lose its shape under water. The corneal endothelium was stained with trypan blue and alizarin red S. Ten BALB/ c (H-2d) mice received a corneal graft taken from a C3H (H-2k) mouse by the underwater technique. The 3.7-mm eye of the BALB/c mouse has a corneal diameter of 3.5 mm. The cornea has a central thickness of 170 microns, the epithelium comprising 30% and the stroma 70%. While none of the corneas separated by the new "underwater" technique evidenced endothelial damage, a 28 +/- 17.0% defect of the endothelial surface was found with the conventional technique. All transplanted corneas were clear when the lids were opened on the 2 postoperative day and clouded between the 7th and 30th days (mean 16.5 days) due to an allograft reaction. The newly developed "underwater" technique is su...Continue Reading

Citations

Aug 1, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·F HoffmannS Bulfone-Paus
Sep 1, 2004·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Er-Ping Zhang, Friedrich Hoffmann
Jun 25, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Svetlana StanojlovicUwe Pleyer
Jan 22, 2002·The British Journal of Ophthalmology·J PlskováJ V Forrester
Nov 21, 2009·Experimental Eye Research·Johanna Tukler HenrikssonJames E Walsh
Aug 2, 2011·European Journal of Immunology·Hongmei FuAndrew J T George
Nov 18, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Felix SchulteFriedrich Hoffmann
Dec 6, 2003·Transplantation·Er-Ping ZhangFriedrich Hoffmann
Oct 25, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Sally HayesKeith M Meek
Mar 23, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephan SchlickeiserBirgit Sawitzki

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