Orthotopic hind-limb transplantation in rats.

Journal of Visualized Experiments : JoVE
Robert SucherGerald Brandacher

Abstract

Composite tissue allotransplantation (CTA) now represents a valid therapeutic option after the loss of a hand, forearm or digits and has become a novel therapeutic entity in reconstructive surgery. However, long term high-dose multi-drug immunosuppressive therapy is required to ensure graft survival, bearing the risk of serious side effects which halters broader application. Further progression in this field may depend on better understanding of basic immunology and ischemia reperfusion injury in composite tissue grafts. To date, orthotopic hind limb transplantation in rats has been the preferred rodent model for reconstructive transplantation (RT), however, it is an extremely demanding procedure that requires extraordinary microsurgical skills for reattachment of vasculature, bones, muscles and nerves. We have introduced the vascular cuff anastomosis technique to this model, providing a rapid and reliable approach to rat hind limb transplantation. This technique simplifies and shortens the surgical procedure and enables surgeons with basic microsurgical experience to successfully perform the operation with high survival and low complication rates. The technique seems to be well suited for immunological as well as ischemia repe...Continue Reading

References

Jun 1, 1978·The Journal of Surgical Research·R I Shapiro, F B Cerra
May 22, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S SchneebergerR Margreiter
Aug 30, 2008·Transplantation·Palmina PetruzzoPedro Cavadas

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Citations

Nov 1, 2011·The Journal of Hand Surgery·Jaimie T ShoresW P Andrew Lee
Dec 17, 2011·Transplantation Proceedings·Z ZhangZ Bi
Apr 17, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Yinan GuoGerald Brandacher
Mar 8, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Samuel A J FidderGerald Brandacher

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