Osa-miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae

The Plant Journal : for Cell and Molecular Biology
Zhaoyun WangHongwei Zhao

Abstract

Exploring the regulatory mechanism played by endogenous rice miRNAs in defense responses against the blast disease is of great significance in both resistant variety breeding and disease control management. We identified rice defense-related miRNAs by comparing rice miRNA expression patterns before and after Magnaporthe oryzae strain Guy11 infection. We discovered that osa-miR164a expression reduced upon Guy11 infection at both early and late stages, which was perfectly associated with the induced expression of its target gene, OsNAC60. OsNAC60 encodes a transcription factor, over-expression of which enhanced defense responses, such as increased programmed cell death, greater ion leakage, more reactive oxygen species accumulation and callose deposition, and upregulation of defense-related genes. By using transgenic rice over-expressing osa-miR164a, and a transposon insertion mutant of OsNAC60, we showed that when the miR164a/OsNAC60 regulatory module was dysfunctional, rice developed significant susceptibility to Guy11 infection. The co-expression of OsNAC60 and osa-miR164a abolished the OsNAC60 activity, but not its synonymous mutant. We further validated that this regulatory module is conserved in plant resistance to multiple...Continue Reading

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Citations

Apr 20, 2019·Journal of Integrative Plant Biology·Zhi-Xue ZhaoWen-Ming Wang
Jul 10, 2019·Genome Génome / Conseil National De Recherches Canada·Wanxia JiangFengxia Liu
Mar 9, 2019·Annual Review of Plant Biology·Xianwei SongYijun Qi
Jan 30, 2020·International Journal of Molecular Sciences·Yanfeng JiaWenxue Zhai
Jan 11, 2020·The Plant Journal : for Cell and Molecular Biology·Lulu QiaoDongdong Niu
Dec 20, 2019·BMC Plant Biology·Ferran Sánchez-SanuySonia Campo
May 21, 2020·The Plant Journal : for Cell and Molecular Biology·Ning JiangYushi Luan
Dec 22, 2019·Journal of Integrative Plant Biology·Shi-Xin ZhouWen-Ming Wang
Jul 5, 2018·International Journal of Molecular Sciences·Siyuan LinHongwei Zhao
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Dec 31, 2020·International Journal of Molecular Sciences·Zhiyuan BianChongying Wang
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Aug 28, 2021·International Journal of Molecular Sciences·Na SongYanyun Hong
Sep 17, 2021·Frontiers in Plant Science·Yan LiWen-Ming Wang
Nov 3, 2021·Plant Biotechnology Journal·Yan LiWen-Ming Wang

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