OsATX1 Interacts with Heavy Metal P1B-Type ATPases and Affects Copper Transport and Distribution

Plant Physiology
Yuanyuan ZhangXingming Lian

Abstract

Copper (Cu) is an essential micronutrient for plant growth. However, the molecular mechanisms underlying Cu trafficking and distribution to different organs in rice (Oryza sativa) are poorly understood. Here, we report the function and role of Antioxidant Protein1 (OsATX1), a Cu chaperone in rice. Knocking out OsATX1 resulted in increased Cu concentrations in roots, whereas OsATX1 overexpression reduced root Cu concentrations but increased Cu accumulation in the shoots. At the reproductive stage, the concentrations of Cu in developing tissues, including panicles, upper nodes and internodes, younger leaf blades, and leaf sheaths of the main tiller, were increased significantly in OsATX1-overexpressing plants and decreased in osatx1 mutants compared with the wild type. The osatx1 mutants also showed a higher Cu concentration in older leaves. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsATX1 interacts with the rice heavy metal P1B-ATPases HMA4, HMA5, HMA6, and HMA9. These results suggest that OsATX1 may function to deliver Cu to heavy metal P1B-ATPases for Cu trafficking and distribution in order to maintain Cu homeostasis in different rice tissues. In addition, heterologous expression of OsAT...Continue Reading

Citations

Jun 30, 2019·International Journal of Molecular Sciences·Ewa Muszyńska, Mateusz Labudda
May 1, 2020·Frontiers in Plant Science·Dujun WangXingming Lian
Oct 27, 2020·Plant Direct·Lauren WhittIvan Baxter
Apr 29, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Anayat Rasool MirShamsul Hayat
Jul 21, 2021·International Journal of Molecular Sciences·Sajad AliHanhong Bae

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