OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.)

Frontiers in Plant Science
Erdong NiChuxiong Zhuang

Abstract

Cuticle waxes, which are primarily comprised of very-long-chain (VLC) alkanes, play an important role in plant reproductive development. ECERIFERUM1 (CER1) is recognized as the core element for VLC alkane biosynthesis in Arabidopsis (Arabidopsis thaliana). However, genes involved in the VLC alkane biosynthesis in rice remain unclear, and the alkane-form pathway in rice has still to be further explored. Here, we show that OsCER1, a homology of CER1, functions in VLC alkanes biosynthesis, which also could regulate anther development and plastids differentiation in rice. OsCER1 was highly expressed in the tapetum (stage 10) and bicellular pollen cells (stage 11). The decreased content of VLC alkanes (C25 and C27) in the OsCER1 knocked down plants as well as the increased content of C27 alkanes in the OsCER1 overexpression plants indicates that OsCER1 participates in VLC alkane biosynthesis. Downregulation of OsCER1 in rice led to sterility, and fewer amyloplasts within the mature pollen grains. In addition, the downregulation of OsCER1 in rice caused delayed tapetal programmed cell death and abnormal development of plastids in the tapetal cells. Furthermore, significantly altered levels of expression of genes involved in the polle...Continue Reading

References

May 5, 2001·Phytochemistry·A M RashotteK. A. Feldmann
May 1, 1995·Plant Physiology·M. A. JenksK. A. Feldmann
Dec 7, 2002·Progress in Lipid Research·Ljerka Kunst, A L Samuels
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Aiming WangGopalan Selvaraj
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·John Shanklin, Edgar B. Cahoon
May 8, 2004·The Plant Cell·R J ScottH G Dickinson
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Apr 4, 2006·Current Opinion in Plant Biology·Christiane Nawrath
Apr 24, 2007·The New Phytologist·Stephen BlackmoreJohn R Rowley
Apr 29, 2008·Trends in Plant Science·Mike PollardJohn B Ohlrogge
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Liên BachJean-Denis Faure
Mar 27, 2009·Journal of Experimental Botany·Zoe A Wilson, Dabing Zhang
Mar 27, 2009·Plant Molecular Biology·Mohammad Asadul IslamLizhong Xiong
Oct 13, 2009·Plant Signaling & Behavior·Donna M BondE Jean Finnegan
Oct 30, 2009·Current Opinion in Plant Biology·Ljerka Kunst, Lacey Samuels
Oct 12, 2010·Plant Signaling & Behavior·Hui Li, Dabing Zhang
Apr 23, 2011·Molecular Plant·Bao-Xiang QinZhu-Kuan Cheng
Sep 21, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Dabing ZhangLu Zhu
Oct 1, 2011·Journal of Integrative Plant Biology·Jun ZhuZhong-Nan Yang
Nov 25, 2011·The Plant Journal : for Cell and Molecular Biology·Jakyung YiGynheung An
Jun 28, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takashi Nobusawa, Masaaki Umeda

Methods Mentioned

BETA
transgenic
PCR
Reverse
transmission electron microscopy
scanning electron microscopy
light microscopy

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