OsHKT1;4-mediated Na(+) transport in stems contributes to Na(+) exclusion from leaf blades of rice at the reproductive growth stage upon salt stress

BMC Plant Biology
Kei SuzukiTomoaki Horie

Abstract

Na(+) exclusion from leaf blades is one of the key mechanisms for glycophytes to cope with salinity stress. Certain class I transporters of the high-affinity K(+) transporter (HKT) family have been demonstrated to mediate leaf blade-Na(+) exclusion upon salinity stress via Na(+)-selective transport. Multiple HKT1 transporters are known to function in rice (Oryza sativa). However, the ion transport function of OsHKT1;4 and its contribution to the Na(+) exclusion mechanism in rice remain to be elucidated. Here, we report results of the functional characterization of the OsHKT1;4 transporter in rice. OsHKT1;4 mediated robust Na(+) transport in Saccharomyces cerevisiae and Xenopus laevis oocytes. Electrophysiological experiments demonstrated that OsHKT1;4 shows strong Na(+) selectivity among cations tested, including Li(+), Na(+), K(+), Rb(+), Cs(+), and NH4 (+), in oocytes. A chimeric protein, EGFP-OsHKT1;4, was found to be functional in oocytes and targeted to the plasma membrane of rice protoplasts. The level of OsHKT1;4 transcripts was prominent in leaf sheaths throughout the growth stages. Unexpectedly however, we demonstrate here accumulation of OsHKT1;4 transcripts in the stem including internode II and peduncle in the repro...Continue Reading

References

Jul 29, 1999·Biophysical Journal·S R DurellH R Guy
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·T HorieA Shinmyo
Dec 18, 2001·Plant Physiology·J F MaM Ichii
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pascal MäserNobuyuki Uozumi
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Blanca GarciadeblásAlonso Rodríguez-Navarro
Sep 13, 2005·Nature Genetics·Zhong-Hai RenHong-Xuan Lin
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology· SunarpiNobuyuki Uozumi
Mar 17, 2006·Plant & Cell Physiology·Tomoaki HorieJulian I Schroeder
Jul 1, 2006·Trends in Plant Science·J Damien PlattenMark Tester
Feb 27, 2007·Plant Physiology·Caitlin S ByrtRana Munns
Feb 28, 2007·Plant, Cell & Environment·Romola Jane DavenportMark Tester
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Brook K NelsonAndreas Nebenführ
May 1, 2008·Annual Review of Plant Biology·Rana Munns, Mark Tester
Sep 29, 2009·Trends in Plant Science·Tomoaki HorieJulian I Schroeder
Mar 13, 2012·Nature Biotechnology·Rana MunnsMatthew Gilliham
Sep 21, 2013·Nature Communications·Naoki YamajiJian Feng Ma
Sep 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J GorhamC N Law
Mar 19, 2014·Trends in Plant Science·Ulrich DeinleinJulian I Schroeder
Jun 24, 2014·Trends in Plant Science·Naoki Yamaji, Jian Feng Ma
Jul 22, 2014·Journal of Integrative Plant Biology·Honghong WuSergey Shabala
Aug 28, 2014·The Plant Journal : for Cell and Molecular Biology·Caitlin Siobhan ByrtMatthew Gilliham
Mar 10, 2015·Journal of Experimental Botany·Paul Rosas-SantiagoOmar Pantoja
Mar 24, 2015·Nature Biotechnology·Hiroki TakagiRyohei Terauchi
Sep 4, 2015·The Plant Journal : for Cell and Molecular Biology·Akimasa SasakiJian Feng Ma

❮ Previous
Next ❯

Citations

Feb 23, 2017·Annual Review of Plant Biology·Abdelbagi M Ismail, Tomoaki Horie
May 11, 2017·The Plant Journal : for Cell and Molecular Biology·Natsuko I KobayashiTomoaki Horie
Dec 12, 2018·Plant, Cell & Environment·Chuanying FangJie Luo
Oct 26, 2018·International Journal of Molecular Sciences·Yu ZhangLongbiao Guo
Jul 31, 2019·Plant & Cell Physiology·Wei-Chen WangYu-Chang Tsai
Sep 8, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sana TounsiFaiçal Brini
Aug 5, 2017·Frontiers in Physiology·Dekoum V M AssahaMahmoud W Yaish
Mar 9, 2018·Frontiers in Plant Science·Khaled M HazzouriKhaled Masmoudi
May 3, 2019·Functional Plant Biology : FPB·Sarin NeangShiro Mitsuya
Jun 15, 2019·Plant & Cell Physiology·Shan FuJixing Xia
Apr 9, 2020·Frontiers in Plant Science·Celymar A SolisZhong-Hua Chen
Nov 23, 2018·International Journal of Molecular Sciences·Min ChenBaoshan Wang
Jan 15, 2021·Current Opinion in Plant Biology·Showkat Ahmad GanieGoetz Hensel
Mar 7, 2021·International Journal of Molecular Sciences·Kimberly S PonceGuoyou Ye
Nov 14, 2020·International Journal of Molecular Sciences·Hua QinRongfeng Huang
Mar 7, 2021·International Journal of Molecular Sciences·Janin RiedelsbergerIngo Dreyer
Mar 16, 2021·Frontiers in Plant Science·Shiyu SunDabing Zhang
Feb 24, 2021·Physiologia Plantarum·Celymar Angela SolisZhong-Hua Chen
Mar 26, 2021·Breeding Science·Ichirou Karahara, Tomoaki Horie

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
confocal microscopy
PCR
in

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.