OsKu70 is associated with developmental growth and genome stability in rice.

Plant Physiology
Jong-Pil HongWoo Taek Kim

Abstract

The cellular functions of Ku70 in repair of DNA double-stranded breaks and telomere regulation have been described in a wide range of organisms. In this study, we identified the rice (Oryza sativa) Ku70 homolog (OsKu70) from the rice genome database. OsKu70 transcript was detected constitutively in every tissue and developmental stage examined and also in undifferentiated callus cells in rice. Yeast two-hybrid and in vitro pull-down experiments revealed that OsKu70 physically interacts with OsKu80. We obtained loss-of-function osku70 T-DNA knockout mutant lines and constructed transgenic rice plants that overexpress the OsKu70 gene in the sense (35S:OsKu70) or antisense (35S:anti-OsKu70) orientation. The homozygous G2 osku70 mutant lines were more sensitive than wild-type plants to a DNA-damaging agent (0.01%-0.05% methyl-methane sulfonate), consistent with the notion that OsKu70 participates in the DNA repair mechanism. Terminal restriction fragment analysis revealed that telomeres in homozygous G2 osku70 mutants were markedly longer (10-20 kb) than those in wild-type plants (5-10 kb), whereas telomere length in heterozygous G2 osku70 mutant and T2 OsKu70-overexpressing transgenic (35S:OsKu70) rice resembled that of the wild-t...Continue Reading

References

Sep 18, 1997·The Journal of Experimental Medicine·H OuyangG C Li
Nov 12, 1998·Trends in Biochemical Sciences·S E Critchlow, S P Jackson
Dec 5, 1998·Biochemical and Biophysical Research Communications·M KoikeT Shiomi
Jul 17, 1999·Trends in Plant Science·V Gorbunova, A A Levy
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·H VogelP Hasty
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J S JeonG An
Sep 14, 2000·FEBS Letters·K SongI Lee
Oct 12, 2000·Molecular Biology of the Cell·P Baumann, T R Cech
Nov 23, 2000·Genes & Development·H L HsuD J Chen
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·M E Gallego, C I White
Jun 28, 2002·Nucleic Acids Research·Chao WeiCarolyn M Price
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Katsunori TamuraHideo Takahashi
Aug 17, 2002·The Plant Journal : for Cell and Molecular Biology·Christopher E WestClifford M Bray
Oct 16, 2002·The Journal of Biological Chemistry·Weihang ChaiJerry W Shay
Jan 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Karel Riha, Dorothy E Shippen
Mar 5, 2003·The Plant Cell·Maria Eugenia GallegoCharles I White
Aug 28, 2003·The Plant Journal : for Cell and Molecular Biology·M E GallegoC I White
Sep 17, 2003·Genes & Development·Anne E StellwagenDaniel E Gottschling
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Michael R Lieber, Zarir E Karanjawala
May 15, 2004·Molecular and Cellular Biology·Kyungjae MyungEric A Hendrickson
Sep 15, 2004·The Journal of Biological Chemistry·Zemfira N KaramyshevaDorothy E Shippen
Oct 20, 2004·Cancer Research·Isabel JacoMaría A Blasco
Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Maria E Gallego, Charles I White
Jul 11, 2006·Annual Review of Genetics·Karel RihaDorothy E Shippen
Sep 23, 2006·Plant & Cell Physiology·Min-Jung HanGynheung An
Sep 23, 2006·The EMBO Journal·Jean-Baptiste VannierMaria Eugenia Gallego
Dec 26, 2006·Molecular and Cellular Biology·J Matthew Watson, Dorothy E Shippen
May 18, 2007·Biochimica Et Biophysica Acta·Pei-Feng LiuRong-Long Pan
Dec 18, 2007·The Plant Cell·Tory ChhunMiyako Ueguchi-Tanaka
May 15, 2008·Biochemical and Biophysical Research Communications·Mi Young ByunWoo Taek Kim
Jul 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yongbao WangEric A Hendrickson

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Citations

Jun 29, 2010·FEBS Letters·J Matthew Watson, Karel Riha
Nov 22, 2015·Biochemical and Biophysical Research Communications·Mi Young ByunWoo Taek Kim
Mar 11, 2015·The Plant Journal : for Cell and Molecular Biology·Martina DvořáčkováJiří Fajkus
Nov 5, 2014·Frontiers in Plant Science·Hiroaki SaikaSeiichi Toki
Jul 26, 2016·Frontiers in Plant Science·Petra Procházková SchrumpfováJiří Fajkus
May 21, 2014·The Plant Journal : for Cell and Molecular Biology·Mi Young Byun, Woo Taek Kim
Apr 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Hansol BaeWoo Taek Kim

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