Osmolarity regulates chondrogenic differentiation potential of synovial fluid derived mesenchymal progenitor cells

Biochemical and Biophysical Research Communications
Karri L Bertram, Roman J Krawetz

Abstract

Cartilage is one of few tissues where adult stem/progenitor cells have not been putatively identified. Recent studies have provided strong evidence that a sub-population of mesenchymal progenitor cells (MPCs) derived from the synovial fluid may be able to affect some degree of cartilage repair both in vivo and in vitro/ex vivo, however this does not appear to be the case in patients with arthritis. Previously, it has been found that synovial fluid osmolarity is decreased in patients with osteoarthritis (OA) or Rheumatoid arthritis (RA) and these changes in osmolarity have been linked to changes in chondrocyte gene regulation. However, it is yet unknown if changes in osmolarity regulate the gene expression in synovial fluid MPCs (sfMPCs), and by extension, chondrogenesis of this cell population. In the present study we have collected synovial fluid samples from normal, OA and RA knee joints, quantified the osmolarity of the fluid and modified the culture/differentiation media to span a range of osmolarities (264-375 mOsm). Chondrogenesis was measured with Alcian blue staining of cultures in addition to quantitative PCR (qPCR) using probes to Sox9, ACAN and Col2A1. Overall, sfMPCs from arthritic joints demonstrated decreased chon...Continue Reading

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Citations

Jan 30, 2015·Journal of Orthopaedic Surgery and Research·Yuelong HuangTiezheng Sun
Apr 9, 2016·PM & R : the Journal of Injury, Function, and Rehabilitation·Gastón Andrés TopolDavid Rabago
Jun 2, 2015·International Journal of Pharmaceutics·Wei Wang
Dec 3, 2017·Applied Biochemistry and Biotechnology·Sorour AhmadyanAli Farazmand
Jan 16, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Behdad PouranHarrie Weinans
Aug 3, 2018·Biological & Pharmaceutical Bulletin·Hisao YamamuraYuji Imaizumi
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May 7, 2015·Molecular Pharmacology·Takashi KuritaYuji Imaizumi
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Michael KittlMartin Jakab
Jan 1, 2021·Biochemical and Biophysical Research Communications·Satoshi YamadaHisao Yamamura
Mar 8, 2021·Acta Biochimica Et Biophysica Sinica·Zhiqin DengWencui Li

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