Osmometric behavior of Drosophila melanogaster embryos

Cryobiology
T T LinP L Steponkus

Abstract

The osmometric behavior of Drosophila melanogaster embryos in permeabilized eggs was studied in a microscope diffusion chamber designed to impose a rapid change in osmotic environment at various temperatures. A numerical model of NaCl diffusion in the chamber predicted that radial variations in concentration arising from the presence of a thin film of solution at the top of the chamber were negligible. On the basis of transient electrical conductance measurements in the chamber, characteristic time constants for the change in concentration averaged over the chamber depth occupied by the eggs were 0.99, 0.77, and 0.60 min at 0, 10, and 20 degrees C, respectively. The chamber response was sufficiently rapid that the characteristic response of the embryo was not masked. Equilibrium volumetric behavior of the embryos indicated that they behaved as nearly ideal osmometers over the range of 0.256 to 2.000 osm, and followed the relation FVeq = 0.123C-1 + 0.541, where FVeq is equilibrium fractional volume and C is osmolality. Nonlinear regression of volumetric data during osmotic contraction yielded an average Lp of 0.722 micron/(min.atm) at 20 degrees C and an apparent activation energy delta E of 8.11 kcal/mol. The coefficients of va...Continue Reading

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Citations

Aug 15, 1997·The Journal of Experimental Zoology·M HagedornW F Rall
Sep 19, 2009·Zebrafish·Vanesa RoblesM Paz Herráez
Aug 24, 2002·Biology of Reproduction·M HagedornB S Pukazhenthi
Oct 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Joseph Saragusty, Amir Arav
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Oct 22, 1998·Biology of Reproduction·M HagedornU Pilatus
May 10, 1990·Nature·P L SteponkusR J MacIntyre
May 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raj R ThakrarAlexander M Seifalian
May 29, 2003·The Journal of Experimental Biology·Xiang-Hong LiuPeter Mazur
Oct 1, 1989·Cryobiology·D V LynchP L Steponkus

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