PMID: 8955405Dec 1, 1996Paper

Osmoprotectants in Halomonas elongata: high-affinity betaine transport system and choline-betaine pathway

Journal of Bacteriology
D CánovasJ J Nieto

Abstract

The osmoregulatory pathways of the moderately halophilic bacterium Halomonas elongata DSM 3043 have been investigated. This strain grew optimally at 1.5 to 2 M NaCl in M63 glucose-defined medium. It required at least 0.5 M NaCl for growth, which is a higher concentration than that exhibited by the H. elongata type strain ATCC 33173. Externally provided betaine, choline, or choline-O-sulfate (but not proline, ectoine, or proline betaine) enhanced the growth of H. elongata on 3 M NaCl-glucose-M63 plates, demonstrating the utilization of these compounds as osmoprotectants. Moreover, betaine and choline stimulated the growth of H. elongata DSM 3043 over the entire range of salinity, although betaine was more effective than choline at salinities below and above the optimum. We found that H. elongata DSM 3043 has at least one high-affinity transport system for betaine (K(m) = 3.06 microM and Vmax = 9.96 nmol of betaine min(-1) mg of protein(-1)). Competition assays demonstrated that proline betaine and ectoine, but not proline, choline, or choline-O-sulfate, are also transported by the betaine permease. Finally, thin-layer chromatography and 13C-nuclear magnetic resonance analysis showed that exogenous choline was taken up and transf...Continue Reading

References

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Citations

May 20, 2005·Extremophiles : Life Under Extreme Conditions·Aharon OrenLaszlo N Csonka
Nov 23, 2006·Molecular Microbiology·Teca Calcagno GalvãoDavid Cánovas
Dec 29, 2010·Current Microbiology·Vida CasaitėRolandas Meškys
Nov 20, 2012·Applied and Environmental Microbiology·Javier Rodríguez-MoyaCarmen Vargas
Mar 13, 2018·FEMS Microbiology Reviews·Nina Gunde-CimermanAharon Oren
Apr 2, 2008·Archives of Microbiology·Regina Spanheimer, Volker Müller
Jun 28, 2016·World Journal of Microbiology & Biotechnology·Mohamed Faraj EdbeibFahrul Huyop
Jul 1, 2017·Extremophiles : Life Under Extreme Conditions·Ya-Hui ShaoWei-Dong Lu
Jun 6, 2018·Journal of Bacteriology·Hind BasbousBruno Franzetti
Nov 20, 2012·Applied and Environmental Microbiology·Adrien Y BurchSteven E Lindow
Apr 26, 2013·The Journal of Biological Chemistry·José M PastorManuel Cánovas
Mar 12, 2011·Die Naturwissenschaften·Francesco Canganella, Juergen Wiegel
Feb 5, 2005·Applied and Environmental Microbiology·Teca Calcagno Galvão, Víctor de Lorenzo
Apr 7, 2010·Applied and Environmental Microbiology·Montserrat ArgandoñaCarmen Vargas
Oct 9, 2003·Applied and Environmental Microbiology·Kathryn J PflughoeftPaula I Watnick
Jan 30, 1999·Applied and Environmental Microbiology·G Nau-WagnerE Bremer
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A VentosaA Oren
Nov 9, 2021·Physical Chemistry Chemical Physics : PCCP·Srijita Paul, Sandip Paul

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