Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice

Microbiology
Arvind A BhagwatAllen Smith

Abstract

We purified osmoregulated periplasmic glucans (OPGs) from Salmonella enterica serovar Typhimurium and found them to be composed of 100 % glucose with 2-linked glucose as the most abundant residue, with terminal glucose, 2,3-linked and 2,6-linked glucose also present in high quantities. The two structural genes for OPG biosynthesis, opgG and opgH, form a bicistronic operon, and insertion of a kanamycin resistance gene cassette into this operon resulted in a strain devoid of OPGs. The opgGH mutant strain was impaired in motility and growth under low osmolarity conditions. The opgGH mutation also resulted in a 2 log increase in the LD50 in mice compared to the wild-type strain SL1344. Inability to synthesize OPGs had no significant impact on the organism's lipopolysaccharide pattern or its ability to survive antimicrobial peptides-, detergent-, pH- and nutrient-stress conditions. We observed that the opgGH-defective strain respired at a reduced rate under acidic growth conditions (pH 5.0) and had lower ATP levels compared to the wild-type strain. These data indicate that OPGs of S. Typhimurium contribute towards mouse virulence as well as growth and motility under low osmolarity growth conditions.

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Citations

Jan 12, 2010·Archives of Microbiology·Liu LiuArvind A Bhagwat
Jan 19, 2013·Current Microbiology·Allen Smith, Arvind A Bhagwat
Jun 27, 2012·Foodborne Pathogens and Disease·Arvind A BhagwatPorteen Kannan
Nov 15, 2011·Journal of Bacteriology·S Betty ZouWilliam Wiley Navarre
Aug 13, 2013·PLoS Genetics·Norbert S HillPetra Anne Levin
Nov 15, 2013·BioMed Research International·Sébastien Bontemps-GalloJean-Marie Lacroix
May 14, 2014·Annual Review of Microbiology·Matthew G Percy, Angelika Gründling
Aug 13, 2015·Environmental Microbiology Reports·Sébastien Bontemps-Gallo, Jean-Marie Lacroix
Jun 9, 2017·EcoSal Plus·Sébastien Bontemps-GalloJean-Marie Lacroix

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