Osmoregulation in the Plotosidae Catfish: Role of the Salt Secreting Dendritic Organ

Frontiers in Physiology
Salman Malakpour KolbadinezhadJonathan M Wilson

Abstract

Unlike other marine teleosts, the Plotosidae catfishes reportedly have an extra-branchial salt secreting dendritic organ (DO). Salinity acclimation [brackishwater (BW) 3aaa, seawater (SWcontrol) 34aaa, and hypersaline water (HSW) 60aaa] for 14 days was used to investigate the osmoregulatory abilities of Plotosus lineatus through measurements of blood chemistry, muscle water content (MWC), Na+/K+-ATPase (NKA) specific activity and ion transporter expression in gills, DO, kidney and intestine. Ion transporter expression was determined using immunoblotting, immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR). HSW elevated mortality, plasma osmolality and ions, and hematocrit, and decreased MWC indicating an osmoregulatory challenge. NKA specific activity and protein levels were significantly higher in DO compared to gill, kidney and intestine at all salinities. NKA specific activity increased in kidney and posterior intestine with HSW but only kidney showed correspondingly higher NKA α-subunit protein levels. Since DO mass was greater in HSW, the total amount of DO NKA activity expressed per gram fish was greater indicating higher overall capacity. Gill NKA and V-ATPase protein levels were greater with HSW...Continue Reading

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Citations

Oct 24, 2018·PloS One·Salman Malakpour KolbadinezhadJonathan M Wilson
Apr 13, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Leighann Martin, Andrew J Esbaugh

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Methods Mentioned

BETA
PCR
glycosylation

Software Mentioned

Leica LAS AF
Multi Gauge
LAS
BLAST
SigmaPlot
ClustalX
4000

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