Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B

Molecular Microbiology
C von BlohnE Bremer

Abstract

Exogenously provided proline has been shown to serve as an osmoprotectant in Bacillus subtilis. Uptake of proline is under osmotic control and functions independently of the known transport systems for the osmoprotectant glycine betaine. We cloned the structural gene (opuE) for this proline transport system and constructed a chromosomal opuE mutant by marker replacement. The resulting B. subtilis strain was entirely deficient in osmoregulated proline transport activity and was no longer protected by exogenously provided proline, attesting to the central importance of OpuE for proline uptake in high-osmolarity environments. The transport characteristics and growth properties of the opuE mutant revealed the presence of a second proline transport activity in B. subtilis. DNA sequence analysis of the opuE region showed that the OpuE transporter (492 residues) consists of a single integral membrane protein. Database searches indicated that OpuE is a member of the sodium/solute symporter family, comprising proteins from both prokaryotes and eukaryotes that obligatorily couple substrate uptake to Na+ symport. The highest similarity was detected to the PutP proline permeases, which are used in Escherichia coli, Salmonella typhimurium a...Continue Reading

Citations

Jan 21, 2003·Mass Spectrometry Reviews·Ran Rosen, Eliora Z Ron
Apr 10, 2007·The Journal of Membrane Biology·H JungD Hilger
Sep 17, 1999·International Journal of Food Microbiology·T Abee, J A Wouters
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J M WoodL T Smith
Jul 31, 2013·Environmental Science & Technology·Aifen ZhouJizhong Zhou
Sep 5, 2001·Molecular Microbiology·C W PriceP Youngman
Feb 16, 2002·Environmental Microbiology·M Roesser, V Müller
Jun 12, 2012·Applied and Environmental Microbiology·Tamara HoffmannErhard Bremer
Nov 13, 2012·Applied and Environmental Microbiology·Adrienne ZaprasisErhard Bremer
Feb 2, 2002·Applied and Environmental Microbiology·Anne U Kuhlmann, Erhard Bremer
Jul 8, 2005·Applied and Environmental Microbiology·Dagmar KapfhammerPaula I Watnick
Jul 31, 2012·Journal of Bacteriology·Kathleen E Fischer, Erhard Bremer
Feb 8, 2011·Journal of Bacteriology·Tamara Hoffmann, Erhard Bremer
Nov 23, 2006·Journal of Bacteriology·Helena B ThomaidesLloyd Czaplewski
Dec 6, 2011·Journal of Bacteriology·Susanne MosesErhard Bremer
Sep 7, 2001·Journal of Bacteriology·A PetersohnM Hecker
Jan 16, 2002·Journal of Bacteriology·Tamara HoffmannErhard Bremer
Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·Carsten HornLutz Schmitt
May 1, 2009·Research in Microbiology·Michael HeckerSusanne Engelmann
Nov 5, 2014·Biological Reviews of the Cambridge Philosophical Society·Yosef FichmanLaszlo N Csonka
Jan 16, 2015·Gut Pathogens·Xiuping FuBiao Kan

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