Osmotic adjustment leads to anomalously low estimates of relative water content in wheat and barley

Functional Plant Biology : FPB
J S BoyerJohn B Passioura

Abstract

Relative water content (RWC) is used extensively to determine the water status of plants relative to their fully turgid condition. However, plants often adjust osmotically to salinity or water deficit, which maintains turgor pressure and obscures the definition of 'full turgidity'. To explore this problem, turgor was measured by isopiestic psychrometry in mature leaf blades of barley (Hordeum vulgare) and durum wheat (Triticum turgidum ssp. durum) salinised to 150 mm NaCl, or bread wheat (Triticum aestivum) grown in soil dehydrated to varying degrees. Osmotic adjustment maintained turgor in all the plants but despite full maintenance in some of the salinised plants, their leaf RWC decreased substantially. This occurred because excess water was absorbed while the samples were floated on water as part of the RWC measurement. The absorption falsely increased the weight of the 'fully turgid' condition, causing RWC to be anomalously low by 10-15%. Cell solution was secreted into intercellular spaces and was seen under a microscope, which is a test encouraged for all RWC measurements. Several alternate methods are suggested for rehydrating tissues while minimising excess water absorption, but no simple definition of 'full turgidity' ...Continue Reading

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Citations

Jul 30, 2010·Journal of Experimental Botany·John S Boyer
Oct 7, 2016·Annals of Botany·S NegrãoM Tester
Dec 1, 2016·Functional Plant Biology : FPB·Rana MunnsTimothy D Colmer
Jul 20, 2010·Journal of Experimental Botany·Mario GutierrezArthur R Klatt
Mar 15, 2011·Journal of Plant Research·Marcel Giovanni Costa FrançaFernando Henrique Aguiar Vale
Jun 14, 2020·Environmental Management·Behnam TashayoMohammad Akbari
Jul 8, 2010·Journal of Experimental Botany·Rana MunnsHamlyn G Jones
Feb 12, 2011·Plant, Cell & Environment·F E RockwellM A Zwieniecki
Jul 4, 2015·Plant, Cell & Environment·Johanna A Bac-MolenaarDick Vreugdenhil
Jun 18, 2020·Frontiers in Plant Science·T E G Alvarez-ArenasEustaquio Gil-Pelegrín
Feb 1, 2017·Functional Plant Biology : FPB·Md HasanuzzamanSergey Shabala
Apr 20, 2016·Trends in Plant Science·Matthew E Gilbert, Viviana Medina

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